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dc.contributor.authorde la Peña, Leobert D.
dc.contributor.authorLavilla-Pitogo, Celia R.
dc.contributor.authorVillar, Corina Belle R.
dc.contributor.authorPaner, Milagros G.
dc.contributor.editorNagasawa, Kazuya
dc.date.accessioned2020-07-22T00:37:03Z
dc.date.available2020-07-22T00:37:03Z
dc.date.issued2005-03
dc.identifier.citationde la Peña, L. D., Lavilla-Pitogo, C. R., Villar, C. B. R., & Paner, M. G. (2005). Prevalence of economically-important viral diseases in wild shrimp (Penaeus monodon) in the Philippines. In K. Nagasawa (Ed.), Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia (pp. 105–114). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.en
dc.identifier.isbn9718511732
dc.identifier.urihttp://hdl.handle.net/10862/5918
dc.description.abstractPrevalences of white spot syndrome virus (WSSV) and monodon baculovirus (MBV) were determined using polymerase chain reaction (PCR) as the detection method in wild black tiger shrimp (Penaeus monodon) collected from seven sampling sites in the Philippines. These seven sampling sites are the primary sources of spawners and broodstocks for hatchery use. Samples for PCR comprised DNA extracts from gills for WSSV detection and from hepatopancreas for MBV. As a result, WSSV was detected in shrimp from all sites except Bohol during dry season, and were negative from all sites except Palawan during wet season. However, all the positive shrimp showed no white spots in the carapace. MBV was also detected from all sites except Palawan, Negros Occidental and Bohol during dry and wet seasons, respectively. The prevalences of WSSV and MBV showed seasonal and sex variations, i.e. the prevalence in dry season (April-May) was higher than in wet season (August-October), and the prevalence in female shrimps was higher than in males. A very low prevalence of dual infection with WSSV and MBV was also observed. These results suggest that the viruses have already established in the local marine environment and in wild populations of P. monodon, and spawners collected during dry season could serve as the main source of contamination for vertical transmission of the virus when they spawn.en
dc.language.isoenen
dc.publisherAquaculture Department, Southeast Asian Fisheries Development Centeren
dc.subjectMBVen
dc.subjectPenaeus monodonen
dc.subjectBaculoviridaeen
dc.subjectPenaeus japonicusen
dc.titlePrevalence of economically-important viral diseases in wild shrimp (Penaeus monodon) in the Philippinesen
dc.typeBook chapteren
dc.citation.spage105en
dc.citation.epage114en
dc.citation.bookTitleRecent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asiaen
dc.subject.asfaWhite spot syndrome virusen
dc.subject.asfaviral diseasesen
dc.subject.asfaanimal diseasesen
dc.subject.asfapolymerase chain reactionen
dc.subject.asfaDNAen
dc.subject.asfanucleic acidsen
dc.subject.asfadisease recognitionen
dc.subject.asfavirusesen
dc.subject.scientificNamePenaeus monodonen


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