02 SEAFDEC/AQD Collaborative Publications: Recent submissions
Now showing items 91-95 of 453
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Marker-aided genetic stock management: prospects in Philippine aquatic biodiversity conservation and aquaculture
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2015)With the advent of DNA marker-based technologies and applications, genetic stock assessment incorporating molecular marker information has become an important tool in managing resources both for aquaculture and stock enhancement. Local initiatives toward this end have been undertaken by several research and academic agencies particularly those with access to advanced molecular genetic laboratory facilities both in the Philippines and in collaborating foreign institutions. Funds coming from the Philippine Department of Science and Technology and/or international research grants have supported work on commercially valuable species such as tilapia, shrimp, mud crabs, abalone, milkfish and some high value marine fishes with a view of utilizing and in the process, demonstrating the significance of more scientific microlevel assessment of stocks. Information drawn from marker-aided genetic stock evaluation can contribute to a better understanding of the impact of how proper stock management can be more effectively achieved and how this method can gradually translate to improved yields both from culture and fisheries. This paper covers a review of the status of this technology as applied to ongoing fish conservation and aquaculture production efforts in the Philippines. -
Updates on the seed production of mud crab
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2015)Widespread interest in mud crab species is increasing because these are highly prized both in domestic and export markets. Among the three mud crab species commonly found in the Philippines, Scylla serrata, S. olivacea, and S. tranquebarica, S. serrata is preferred by farmers because it is larger and less aggressive than the other species. Likewise, S. serrata is the most widely distributed species in the Indo-west Pacific region. Hatchery-produced seedstock are presently used by some crab farmers in their grow-out operations. In the hatchery phase, feeding mud crab larvae with shrimp formulated diets and natural food was found to reduce the occurrence of molt death syndrome, one of the major problems in seed production. Larvae given 25% formulated diet (FD) + 75% natural food (NF; rotifers and Artemia) and 50% FD + 50% NF showed better performance than those larvae fed 100% FD, 100% NF and 75% FD + 25% NF indicating that usage of natural food, especially the expensive Artemia, can be reduced. Since the early crab instar (C) produced in the hatchery need to be grown further before stocking in grow-out ponds, two phases of nursery culture have been developed. C1-2 are grown to 1.5-2.0 cm carapace width (CW) size in the first phase and further grown to 3.0-4.0 cm CW in the second phase. Nursery rearing is done in net cages installed in ponds for easy retrieval. A combination of mussel or trash fish and formulated diet is used as feed. Domestication of the mud crab S. serrata as a prerequisite to selective breeding has been done at SEAFDEC/AQD. Likewise, defining criteria for the determination of quality of newly hatched zoeae for stocking in the hatchery was initiated. Newly hatched zoeae were subjected to starvation and stress test using formalin. Starvation failed to elicit responses that were significantly different between the good and poor quality larvae hence it is not suitable for larval quality evaluation. Based on three-year data, the formalin stress test gave mean cumulative mortalities of 2.38±0.32, 8.24±0.88, 20±1.58 in good quality larvae, and 43.74±2.39 while 22.93±4.19, 63.68±7.17, 84.29±3.88 and 97.65±1.06 for poor quality larvae at 0 (control), 20, 30 and 40 ppm formalin, respectively. As formalin level increased, cumulative larval mortality also increased regardless of the quality of the larvae. Formalin stress test proved to be a reliable method to determine whether a batch of newly hatched zoeae was of good or poor quality. -
Feed formulation for sustainable aquaculture
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2015)As aquaculture production of tropical fish and crustacean species becomes more intensified, practical diets need to be formulated to be cost effective and environment-friendly. Ingredients should be included to satisfy the nutrient requirements of the animal, promote optimal fish growth, and boost the income of small-scale farmers and commercial producers with minimal impacts to the surrounding environment. Feed formulation for sustainable aquaculture should aim at increasing aquaculture system performance and profitability, enhancing the animals disease resistance, increasing attractability, palatability, and digestibility of practical diets, and maintaining environmental quality through sound feeding management and good aquaculture practices. More vigorous research and development efforts need to be supported to generate feed technologies that will ensure a steady and reliable supply of safe and high quality aquaculture products to the public while preserving the environment. -
Potential of cowpea (Vigna unguiculata L.) meal as an alternative protein source in diets for giant freshwater prawn (Macrobrachium rosenbergii, de Man 1879)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2015)Growth trials were conducted to evaluate cowpea Vigna unguiculata (L.) meal as a potential protein source in diets for giant freshwater prawn, Macrobrachium rosenbergii (de Man 1879), reared in tank and lake-based cages. Five isonitrogenous (approximately 37% crude protein) and isocaloric diets were formulated where fish meal (FM) protein was replaced with 0%, 15%, 30%, 45% and 60% cowpea meal protein (or CP0, CP15, CP30, CP45, and CP60, respectively). Results of an 8-week tank trial showed that the final body weight (FBW), percent weight gain, specific growth rate (SGR) and survival of prawns were not significantly influenced by dietary treatments (P > 0.05), although the highest values, except for survival, were observed with CP45. In a lakebased cage trial that lasted for 16 weeks, prawns fed CP30 and CP45 had significantly higher FBW (13.1 and 14.4 g, respectively) compared to other treatment groups (P < 0.05). SGR (4.52 5.00%/ day), survival rates (53-77%), yield (98.5-116.5 g m-2) and feed conversion ratio (FCR; 2.0-2.7) were not affected by increasing levels of cowpea meal in the diets. Based on these results, cowpea meal can be considered as an alternative protein source in diets for M. rosenbergii. -
Utilization of sensors and SMS technology to remotely maintain the level of dissolved oxygen, salinity and temperature of fishponds
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2015)Due to the occurrence of fish kills in various fish producing areas in our country, millions of pesos and opportunities for the Filipino people had been put into waste. Bataan Peninsula State University (BPSU) collaborated with the Central Luzon Association of Small-scale Aquaculture to devise strategies to address the said problem and prevent further losses. More often than not, a fish kill can be attributed to the low level of dissolved oxygen (DO) in the water, decrease or increase in salinity and sudden increase in temperature, which usually occur after heavy rainfall, flooding or high tide, or high levels of ammonia due to decomposing organic matter and high temperature during summer. For these reasons, BPSU researchers tested the use of radio frequencies and installed sensors in different areas of the fishpond at various depths to remotely monitor the levels of DO, salinity and temperature of the water. Once these reach critical levels, the installed system which comes with a specific program, will send an alarm through radio frequencies via Short Messaging Services (SMS) technology on the cellular/mobile phone of the caretaker or the fishpond operator. Upon receiving the alarm, caretakers were able to adjust the levels of dissolved oxygen, salinity and temperature of the water by remotely switching on the air compressor or the electric water pump using their cellular/ mobile phone, thus preventing losses due to fish kills.






