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GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis

dc.contributor.authorGallardo, Wenresti G.
dc.contributor.authorHagiwara, Atsushi
dc.contributor.authorHara, Kenji
dc.contributor.authorSoyano, Kiyoshi
dc.contributor.authorSnell, Terry W.
dc.date.accessioned2014-01-06T08:47:41Z
dc.date.available2014-01-06T08:47:41Z
dc.date.issued2000
dc.identifier.citationGallardo, W. G., Hagiwara, A., Hara, K., Soyano, K., & Snell, T. W. (2000). GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 127(3), 301-307.en
dc.identifier.issn1095-6433
dc.identifier.urihttp://hdl.handle.net/10862/1870
dc.description.abstractγ-Aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) have been shown to increase the reproduction of the Brachionus plicatilis (NH3L strain). In the present study, the endogenous presence of GABA and 5-HT in the rotifers B. plicatilis (NH3L and Kamiura strains) and Brachionus rotundiformis (Langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (HPLC). HPLC showed that GABA and 5-HT concentrations in the three rotifer strains range from 71 to 188 pmol/mg and from 12 to 64 pmol/mg, respectively. A total of 33 amino acids were also detected in B. plicatilis and B. rotundiformis, with glutamic acid, serine, glycine, taurine, threonine, alanine, arginine, proline, valine and isoleucine in high concentrations relative to other amino acids.en
dc.description.sponsorshipThis study was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (No. 10660187) and Integrated Research Program for Effects of Endocrine Disrupters on Agriculture, Forestry, and Fisheries and their Action Mechanisms on Domestic Animals and Fishes (ED-99-II-3-1).en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectBrachionus plicatilisen
dc.subjectBrachionus rotundiformisen
dc.subjectDot blot immunoassayen
dc.subject5-HTen
dc.subjectgamma-aminobutyric aciden
dc.subjectRotiferaen
dc.subjectserotoninen
dc.titleGABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformisen
dc.typeArticleen
dc.identifier.doi10.1016/S1095-6433(00)00266-X
dc.citation.volume127
dc.citation.issue3
dc.citation.spage301
dc.citation.epage307
dc.citation.journalTitleComparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiologyen
seafdecaqd.library.callnumberVF SJ 0651
seafdecaqd.databank.controlnumber2000-34
dc.subject.asfaamino acidsen
dc.subject.asfabiochemical compositionen
dc.subject.asfareproductive cycleen
dc.subject.asfaHPLCen
dc.subject.asfaneurotransmittersen
local.subjectAmino acidsen
local.subjectBrachionusen
local.subjectDot blot immunoassayen
local.subject5-HTen
local.subjectGABAen
local.subjectHPLCen
local.subjectNeurotransmittersen
local.subjectRotiferaen
local.subjectSerotoninen


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    These papers were contributed by Department staff to various national and international journals.

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