Now showing items 141-160 of 1267

    • Dietary taurine in practical diet for two sizes of snubnose silver pompano, Trachinotus blochii 

      Mamauag, Roger Edward ORCID; Catacutan, Mae R.; Coloso, Relicardo M.; Erazo-Pagador, Gregoria; Mallare, Mae F.; Ragaza, Janice (Wiley, 2021-09-27)
      Two separate feeding experiments determined taurine requirement levels in diets for growth of two sizes of snubnose silver pompano. The first experiment was conducted for 14 weeks using pompano with average weight of 0.4 ± 0.01 g. Experimental diets were isonitrogenic, isolipidic and isoenergetic. Inclusion of 0.346% taurine in diet served as control, while five diets were with graded taurine levels at 0.5 (0.846%), 1.0 (1.346%), 1.5 (1.846%), 2.0 (2.346%) and 2.5 (2.846%) %. Three hundred and sixty pompano fishes were randomly distributed to experimental tanks (250-L, 15 fish per tank) in quadruplicate. Pompano fed diets with 1.0% taurine had significantly higher (\(p\) < .05) specific growth rate, per cent body weight gain (PWG) and feed utilization efficiency. The second experiment was conducted for 17 weeks using 104 ± 1.82 g pompano. Similar treatments were used except for diets with 0.5% and 2.5% taurine. Fifteen pompano were stocked in floating net cages in triplicate. Quadratic regression analysis of PWG and taurine levels revealed that pompano weighing 0.4 g required 1.55 g taurine 100 g-1 diet (0.352 g taurine 100 g-1 protein) while larger-sized pompano weighing 104 g required 0.937 g taurine 100 g-1 diet (0.213 g taurine 100g-1 protein).
    • Ice-Ice disease: An environmentally and microbiologically driven syndrome in tropical seaweed aquaculture 

      Ward, Georgia; Kambey, Cicilia; Faisan, Joseph P., Jr. ORCID; Tan, Pui Ling; Daumich, Caroline; Matoju, Ivy; Stentiford, Grant; Bass, David; Lim, Phaik Eem; Brodie, Juliet; Poong, Sze Wan (Wiley, 2022-01)
      Seaweeds account for nearly 30% of global aquaculture production by volume, and their cultivation provides important opportunities for employment and wealth generation, particularly in rural coastal communities. Eucheumatoids (Kappaphycus and Eucheuma spp.) are tropical red algae commercially cultivated as raw material for production of carrageenans, particularly in South-East Asia and the Western Indian Ocean region. Diseases and pests are major limiting factors in eucheumatoid production, particularly a condition known as ‘ice-ice’ disease (IID). IID is characterized by bleaching of the thallus followed by the disintegration of affected tissues, leading to a loss of biomass and reduced carrageenan yield. IID has been associated with unfavourable changes in environmental conditions, particularly an increase in sea water temperature and a decrease in salinity, and infection by opportunistic or pathogenic bacteria. However, given that no single or combined causal agents have been definitely identified, IID may be considered a complex pathobiotic syndrome in which multiple factors contribute to the development of disease signs. Here, we review current knowledge of the abiotic and biotic factors associated with the development of IID, and the strategies employed to mitigate economic losses resulting from disease outbreaks. We discuss syndromic diseases in other marine holobionts, particularly other red algae and corals, in relation to their similarity to IID, and suggest the application of comprehensive and complementary methodologies, including high-throughput sequencing and high-quality microscopy, for enhancing our understanding of the pathological and microbiological processes associated with IID signs.
    • Performance, digestibility efficiencies, gut and liver morphologies of snub nose pompano Trachinotus blochii fed spray‐dried haemoglobin meal as protein substitute for fishmeal 

      Mamauag, Roger Edward ORCID; Ragaza, Janice; Nacionales, Trisha J. (Wiley, 2021-07-12)
      Spray-dried haemoglobin meal, a by-product and derivative of the swine processing industry, is formed when haemoglobin is separated hygienically from pig's blood. A 120-days feeding trial using snub nose pompano Trachinotus blochii (1.01 ± 0.02 g) tested spray-dried haemoglobin meal (SHM) as an ingredient in six iso-nitrogenous (crude protein of 50%) and iso-lipidic (crude fat of 8%) diets, namely, a diet with 0% SHM (control) and five diets replacing fishmeal with SHM at 4, 8, 12, 16 and 20%. Weight gain percentage (WG%) significantly decreased (p < 0.05) when fish were fed diets with 12, 16 and 20% SHM inclusion levels. Feed conversion ratio and survival significantly decreased (p < 0.05) when fish were fed diets with 16 and 20% SHM inclusion levels. However, visceral somatic index, hepatosomatic index, condition factor and proximate composition were not affected (p > 0.05) by SHM inclusion levels. As an ingredient for pompano feed, SHM has digestibilities for protein, lipid and dry matter at 89, 78 and 45% respectively. No apparent pathological changes were observed in the intestines. However, physiological changes in the liver have been seen in fish fed diets containing 12, 16 and 20% of SHM. Based on the quadratic regression model at the maximum response of weight gain percentage, the optimum inclusion level of SHM was estimated to be 3.08% (5.87% SHM protein in the diet).
    • Flakes versus noodles as feed for the abalone Haliotis asinina, does form matter? 

      Lebata-Ramos, Ma. Junemie Hazel ORCID; Binas, Joseph; Solis, Ellen Flor; Balinas, Vicente T. (Wiley, 2021-12)
      This paper reports the first use of thin flakes (310–315 μm thick) formulated diets as feed for the abalone Haliotis asinina. The study compared noodles and flakes prepared using refined and unrefined diets developed earlier for H. asinina (Bautista-Teruel et al., J. Shellfish Res., 35, 2016, 633). Four treatment diets in triplicates (refined flakes, MBTr-F; refined noodles, MBTr-N; unrefined flakes, MBTur-F; and unrefined noodles, MBTur-N) were used in a feeding experiment conducted in a tank facility. After three months, abalone fed MBTr-F had significantly higher mean shell length and body weight (35.92 ± 0.36 mm, 9.24 ± 0.29 g) compared with those fed MBTr-N (34.63 ± 0.37 mm, 8.22 ± 0.28 g) and MBTur-N (34.23 ± 0.36 mm, 7.66 ± 0.26 g), but did not significantly differ with those fed MBTur-F (35.40 ± 0.30 mm, 8.57 ± 0.24 g). Likewise, abalone fed MBTr-F had significantly higher mean growth rates (5.31 ± 0.08 mm, 2.59 ± 0.06 g/month), compared with those fed MBTr-N (4.90 ± 0.19 mm, 2.26 ± 0.12 g/month) and MBTur-N (4.71 ± 0.02 mm, 2.07 ± 0.06 g/month), but did not significantly differ with those fed MBTur-F (5.11 ± 0.06 mm, 2.37 ± 0.04 g/month). Survival (96.67–98.89%) and FCR (2.2–2.5) did not significantly differ among treatment diets. Between the flaked diets, MBTur-F is the cheaper option. However, the use of this unrefined diet resulted in distinct turquoise abalone shell colour, not observed in those fed MBTr-F.
    • Comparative transcriptome profiling of heat stress response of the mangrove crab Scylla serrata across sites of varying climate profiles 

      Shrestha, Anish ORCID; Lilagan, Crissa Ann I.; Guiao, Joyce Emlyn B.; Romana-Eguia, Maria Rowena R. ORCID; Ablan Lagman, Ma. Carmen (BioMed Central, 2021)
      Background: The fishery and aquaculture of the widely distributed mangrove crab Scylla serrata is a steadily growing, high-value, global industry. Climate change poses a risk to this industry as temperature elevations are expected to threaten the mangrove crab habitat and the supply of mangrove crab juveniles from the wild. It is therefore important to understand the genomic and molecular basis of how mangrove crab populations from sites with different climate profiles respond to heat stress. Towards this, we performed RNA-seq on the gill tissue of S. serrata individuals sampled from 3 sites (Cagayan, Bicol, and Bataan) in the Philippines, under normal and heat-stressed conditions. To compare the transcriptome expression profiles, we designed a 2-factor generalized linear model containing interaction terms, which allowed us to simultaneously analyze within-site response to heat-stress and across-site differences in the response. Results: We present the first ever transcriptome assembly of S. serrata obtained from a data set containing 66 Gbases of cleaned RNA-seq reads. With lowly-expressed and short contigs excluded, the assembly contains roughly 17,000 genes with an N50 length of 2,366 bp. Our assembly contains many almost full-length transcripts – 5229 shrimp and 3049 fruit fly proteins have alignments that cover >80% of their sequence lengths to a contig. Differential expression analysis found population-specific differences in heat-stress response. Within-site analysis of heat-stress response showed 177, 755, and 221 differentially expressed (DE) genes in the Cagayan, Bataan, and Bicol group, respectively. Across-site analysis showed that between Cagayan and Bataan, there were 389 genes associated with 48 signaling and stress-response pathways, for which there was an effect of site in the response to heat; and between Cagayan and Bicol, there were 101 such genes affecting 8 pathways. Conclusion: In light of previous work on climate profiling and on population genetics of marine species in the Philippines, our findings suggest that the variation in thermal response among populations might be derived from acclimatory plasticity due to pre-exposure to extreme temperature variations or from population structure shaped by connectivity which leads to adaptive genetic differences among populations.
    • The information-seeking behavior and levels of knowledge, precaution, and fear of college students in Iloilo, Philippines amidst the COVID-19 pandemic 

      Superio, Daryl ORCID; Anderson, Kristen; Oducado, Ryan Michael; Luceño, Myrna; Palcullo, Vince; Bendalian, Maria Vanessa (Elsevier, 2021-06-24)
      COVID-19 pandemic is devastating the health, social, and economic well-being of citizens worldwide. The high rates of morbidity and mortality and the absence of vaccines cause fear among the people regardless of age, gender, or social status. People's fear is heightened by misinformation spread across all media types, especially on social media. Filipino college students are one of the top Internet users worldwide and are very active in social media. Hence they are very prone to misinformation. This paper aims to ascertain the levels of knowledge, precaution, and fear of COVID-19 of the college students in Iloilo, Philippines, and determine the effects of their information-seeking behavior on the variables above. This paper is a cross-sectional survey that used a qualitative-quantitative method and snowball sampling technique. Data were gathered among 228 college students using an online survey instrument a few months after the pandemic began. College students were knowledgeable of the basic facts about the highly infectious COVID-19. However, the majority were inclined to believe the myths and misinformation regarding the pandemic. Television was the primary, most believable, and preferred source when seeking information. The Internet as a preferred source of information was significantly associated with a high level of knowledge. In contrast, the information sourced from interpersonal channels were found to make college students very cautious. The local presence of COVID-19 cases had caused college students to fear, likely exacerbated by the plethora of information about the pandemic, mostly from Facebook. This is the first study conducted on the effects of the information-seeking behavior on the levels of knowledge, precaution, and fear of COVID-19 of the college students in Iloilo, Philippines.
    • Occurrence and disappearance of a non-native goby Rhinogobius sp. OR in relation to hydrological conditions in the Kamo River, southwestern Japan 

      Ito, Sayaka ORCID; Tamura, Yoshinobu; Sato, Akira; Onishi, Hidejiro; Shibuya, Masaki; Uchida, Yuki; Inoue, Mikio; Omori, Koji (Springer, 2022-01)
      We monitored the abundance of a non-native (Rhinogobius sp. OR) and two native stream gobies (R. fluviatilis and R. nagoyae) over a decade, from 1995 to 2004, in a fixed reach of a tributary of the Kamo River, southwestern Japan. The non-native Rhinogobius sp. OR appeared and reproduced in 1997, when an increased deposition of fine sediments occurred. However, after a severe spate in 1999, the non-native goby almost completely disappeared from the study reach, while the density of the native R. fluviatilis increased. The severe spate is likely to have prevented the establishment of the non-native goby. Our results provide an example of abiotic barriers to invasion.
    • Producing young, single and meaty oyster Crassostrea iredalei (Faustino, 1932) in grow‐out culture using pouches suspended from rafts 

      Lebata-Ramos, Ma. Junemie Hazel ORCID; Dionela, Cleresa S.; Novilla, Schedar Rose M.; Sibonga, Rema ORCID; Solis, Ellen Flor; Mediavilla, Jonas P. (Wiley, 2021-06-11)
      Growth and survival of the slipper oyster Crassostrea iredalei using traditional (tires and oyster shells) and new methods (pouches and trays) suspended from floating rafts were compared. After six months of grow-out culture, mean shell length (mm SL) and body weight (g BW) of oysters were significantly highest in those reared in pouches (89.32 ± 0.41 mm SL, 87.70 ± 0.70 g BW), followed by those in trays (83.16 ± 0.31 mm SL, 87.06 ± 0.72 g BW), then those harvested from oyster shells (76.42 ± 0.88 mm SL, 67.35 ± 1.03 g BW), and lastly from tires (72.30 ± 1.21 mm SL, 55.07 ± 1.49 g BW). Growth rates both for length and weight were almost comparable between oysters reared in pouches (6.05 ± 1.28 mm mo−1, 10.98 ± 2.05 g mo−1) and trays (5.01 ± 1.46 mm mo−1, 10.77 ± 2.58 g mo−1). Although 60.7% of the oysters harvested from pouches (n = 1071) were classified as large (85–100 mm SL) and jumbo (100–120 mm SL) and only 39.5% of those from trays (n = 1048), their meat yield did not significantly differ (pouches = 22.60 ± 0.86%; trays = 24.76 ± 0.93%). Survival in pouches (95.2 ± 0.77%) and trays (93.15 ± 2.10%) was almost parallel at harvest. Growing oysters using the new methods produced single, larger, meatier oysters of almost the same size at a shorter culture duration.
    • Single and mixed species of microalgae as larval food for the tropical sea cucumber Holothuria scabra 

      Sibonga, Rema ORCID; Laureta, Liberato V.; Lebata-Ramos, Ma. Junemie Hazel ORCID; Nievales, Marie Frances J.; Pedroso, Fiona L. (Springer, 2021-05-31)
      Different microalgae as larval food for Holothuria scabra, a high-value sea cucumber species with hatchery technology continually being refined to improve production, were studied. Two experiments were conducted to determine the performance of the larvae—first, the use of single-algal diet of either Chaetoceros calcitrans, Rhodomonas sp., or Tetraselmis tetrathele as feed; and second, the use of a combination of two algal species, C. calcitrans + Rhodomonas sp., C. calcitrans + T. tetrathele, or Rhodomonas sp. + T. tetrathele as feed. For both experiments, hatched larvae at 2 days post-fertilization (DPF) were used for initial stocking at a density of 200 larvae L−1. Growth, development, and survival were monitored until more than 50% of the larval population were considered as competent larvae (late auricularia). In the first experiment, during the early stage of development (early aricularia), larvae fed Rhodomonas sp. were larger compared to other treatments. However, when the larvae reached middle and late auricularia, they performed better when fed with C. calcitrans. Those larvae fed with C. calcitrans were significantly larger and showed faster development to competent larvae. In the second experiment, larvae fed mixed C. calcitrans + Rhodomonas sp. showed better performance in growth, development, and survival. The present study showed that C. calcitrans, when used solely as feed or in combination with Rhodomonas sp., promoted better growth, development, and survival of H. scabra larvae.
    • Effects of weaning on survival and growth of silver therapon (Leiopotherapon plumbeus) larvae fed live and artificial diet 

      Aya, Frolan ORCID; Nillasca, Vicar Stella N.; Garcia, Luis Maria ORCID (Wiley, 2021-10)
      This study examined the effects of early weaning strategies on growth and survival of the silver therapon Leiopotherapon plumbeus larvae. In Trial 1, four larval age groups (8, 14, 20 and 26 days post‐hatch (dph)) were abruptly weaned to commercial powdered tilapia diet and compared with wild copepod‐fed group as the control. In Trial 2, another batch of three weaning age groups were subjected to gradual weaning (8 dph larvae with 8 days co‐feeding, 14 dph larvae with 6 days co‐feeding and 20 dph larvae with 4 days co‐feeding) with Artemia nauplii prior to weaning on commercial powdered prawn diet and compared with a control group consisted of larvae fed exclusively on Artemia nauplii. Both weaning trials were conducted for 21 days. A gradual weaning strategy promoted better survival (22.2%–40.0%) among weaning age groups than the abrupt weaning strategy (2.2%–36.7%). Except for those weaned at 26 dph, abrupt weaning resulted in significantly poor growth and survival among all weaning age groups compared with the wild copepod‐fed control group. The highest survival and growth were observed in 20 dph larvae with 4 days live food co‐feeding, although the values were significantly lower than the Artemia‐fed control group. These results suggest that abrupt weaning at 26 dph, and gradual weaning for 4 days from 20 dph promote successful rearing of silver therapon larvae and that a microparticulate diet specifically formulated for this species needs to be developed.
    • Preliminary survey of pests and diseases of eucheumatoid seaweed farms in the Philippines 

      Faisan, Joseph P., Jr. ORCID; Luhan, Maria Rovilla; Sibonga, Rema ORCID; Mateo, Jonalyn; Ferriols, Victor Marco Emmanuel; Brakel, Janina; Ward, Georgia M.; Ross, Stuart; Bass, David; Stentiford, Grant; Brodie, Juliet; Hurtado, Anicia Q. (Springer, 2021-05-04)
      Farmed eucheumatoids are the top aquaculture commodity in the Philippines, contributing more than 60% of total national aquaculture production by volume. Despite significant production losses observed in recent years due to pests and diseases, data relating to the temporal and spatial geographic distribution, seasonality, prevalence, and etiology of these critical problems at the farm level are lacking. Here, we assessed the prevalence of pests and diseases at 16 farm sites within several major seaweed growing areas, including Luzon (Palawan), Visayas (Bohol), and Mindanao (Zamboanga City, Davao del Norte, and Tawi-Tawi). Prevalence of pests and diseases were recorded by assessment of individual seaweed plants in situ, comprising ≥5% of the total number of seaweed plants on each farm. Results from this survey revealed “ice-ice” disease (IID) at all sites, albeit at low prevalence rates of below 25%, the exception being one site where prevalence was 100%. No significant difference in the prevalence of IID was found between farms in shallow (≈0.5 m during low tide) and deep water sites (depths exceeding 4 m during the lowest tide) (p>0.05). Moderate prevalence (26–50%) of the macro-epiphyte pests Sargassum and Ulva was reported in Luzon. Further, epiphytic filamentous algae (EFA), black spots, and evidence of grazing were recorded in several farms with low prevalence (0.2–11.2%). The three farms with the highest prevalence of IID and EFA were evaluated to determine the severity of cover on the thallus of infected plants. A very high severity of IID (76–100% coverage) was observed on the secondary and primary branches, while for EFA, low severity (≤25%) was found in all regions of the thallus. Our results suggest the widespread occurrence of yield-limiting diseases and pests affecting eucheumatoid farms in the Philippines. Strategies for the diagnosis, surveillance, and management of yield-limiting pests and diseases are needed to ensure maintenance and sustainable expansion of this important aquaculture sector in the Philippines.
    • Internet use, eHealth literacy and fear of COVID-19 among nursing students in the Philippines 

      Oducado, Ryan Michael; Tuppal, Cyruz; Estoque, Homelo; Sadang, Jonaid; Superio, Daryl ORCID; Real, Don Vicente; Roa, Mary Nellie; Malaga, Xerxes; Quiros, Jejomar; Fajardo, Maria Teresa; Dela Rosa, Ronnell (Pablo de Olavide University, 2021-05-03)
      The COVID-19 outbreak situation brought into focus not only the safety but also the mental wellbeing of nursing students. At present, the level of fear of COVID-19 and associated factors among nursing students in the Philippines is not well understood. This cross-sectional online survey determined the relationship between Internet use and eHealth literacy with fear of COVID-19. One thousand three hundred and sixty-seven (n=1,367) answered an online survey using the adopted eHealth Literacy Scale (eHEALS), and Fear of COVID-19 Scale (FCV-19S) administered from May 1 to 15, 2020. Descriptive statistics, tests for differences, and correlational analysis were performed. Results indicated that the composite score of the FCV-19S was 3.65, indicating moderate to high levels of fear. Fear of COVID-19 significantly differed based on sex, year level, and location. A significant inverse relationship was found between the average daily use of the Internet and fear of COVID-19. On the other hand, no significant association was noted between eHealth literacy and fear of COVID-19. The first wave of the COVID-19 outbreak has resulted in a far-reaching impact on nursing students' psychological wellbeing. This study highlights the value of the Internet and its use during the outbreak may not always lead to higher fear related to COVID-19. Nursing schools may need to create strategies to promote regulated and responsible Internet use, address students' mental health concerns and develop interventions to respond proactively to mitigate or reduce fear among nursing students during the pandemic.
    • Nursery and grow-out culture of the abalone Haliotis asinina on a reef flat: A comparison of growth and survival using different culture containers 

      Lebata-Ramos, Ma. Junemie Hazel ORCID; Mediavilla, Jonas P.; Solis, Ellen Flor; Sibonga, Rema ORCID; Alicante, Frances Patrick L.; Dionela, Cleresa S. (Elsevier, 2021-08)
      The study was conducted to compare four different culture containers [cage, recycled oil container, tray (control), and tube] in the nursery and grow-out culture of the abalone Haliotis asinina on a reef flat. For nursery culture, early juvenile abalone with mean shell length (SL) of 1.30 ± 0.006 cm and mean body weight (BW) of 0.45 ± 0.007 g were stocked in the containers at 100 ind m−2 and reared until they attained the 3 cm SL size required for grow-out culture in three of the four culture containers. For grow-out culture, juvenile abalone harvested from nursery culture with mean SL of 3.23 ± 0.018 cm and mean BW of 7.33 ± 0.131 g were stocked in the containers at 50 ind m−2 and reared until they were harvestable at 5 cm SL cocktail size in three of the four culture containers. The 3 cm SL grow-out size was attained at 90 days of culture (DOC) in recycled containers, trays, and tubes, while the 5 cm SL cocktail size at 180 DOC in the same containers. Abalone reared in tubes had the highest mean SL after 90 DOC in nursery culture (3.21 cm), while those in trays after 180 DOC in grow-out culture (5.30 cm). However, at the end of the nursery and grow-out culture, there were no significant differences in mean SL among the abalone reared in these three culture containers. In both culture phases, abalone reared in cages had the lowest mean SL, 2.88 and 4.44 cm, respectively. Survival was highest in trays (98.64%) during nursery culture and in tubes (96.57%) during grow-out culture. With comparable results in recycled containers, trays, and tubes for both growth and survival at the nursery and grow-out phases, tubes are recommended for use when culture is to be done on reef flats. Tubes are the most stable and durable among the four culture containers tested. They can withstand strong winds and waves and may be reused for several culture runs.
    • Factors affecting the spatio-temporal variability in the production of sandfish Holothuria scabra juveniles in floating hapa ocean nursery systems 

      Altamirano, Jon ORCID; Sinsona, Minin ORCID; Caasi, Olivier Josh ORCID; de la Torre-de la Cruz, Margarita; Uy, Wilfredo ORCID; Baylon, Roselyn ORCID; Juinio-Meñez, Marie Antonette ORCID (Elsevier, 2021-04)
      Hatchery production of the tropical sea cucumber Holothuria scabra or sandfish has been successfully demonstrated in various countries, especially during the past decade. However, the main bottleneck in up-scaling production of larger sandfish juveniles is in the nursery phase where land-based facilities are limited in the Philippines compared to other countries. This has led to some promising pioneering research in ocean-based sandfish nurseries in the Philippines. However, site-specific and seasonal inconsistencies in production levels were observed. In this study, a comprehensive experiment comparing 17 replicated nursery culture operations of sandfish juveniles using floating hapas, was conducted across four sites with distinct geo-climatic zones in the Philippines and at different months from 2013 to 2016. We evaluated the performance of the ocean-based floating hapa nursery system by determining the effects of periphyton characteristics and broad-scale differences in meteorological variables to growth and survival of juvenile sandfish within different geo-climatic zones. Our data confirmed high spatial (across site) and temporal (among trials within site) variability in sandfish culture performances. Wind stress was the most significant driver of this variation, which negatively influenced growth and survival of juveniles especially during Cycle 1 (first 30 d). Sandfish growth was positively related to Chl-a and mean temperature, but was negatively impacted by Wind stress, Autotropic Index (AI), total rainfall (∑Rain), and sea-surface atmospheric pressure; while survival was positively affected by phaeophytin and Ash-Free Dry Weight (AFDW) content of the hapa biofilm. In Cycle 2 (succeeding 31–60 d), growth and survival was greatly influenced negatively by the re-stocked density (number of individuals surviving from Cycle 1), while survival was further affected negatively by ∑Rain and AFDW. Our results provide significant information on suitable ocean nursery conditions, periphyton/biofilm characteristics, and spatial-temporal considerations for optimizing production of H. scabra juveniles in floating hapas. We also highlight the negative effects of increasing biomass density when on-growing larger juveniles in this system. These insights are valuable in optimizing management and operation of ocean nurseries for sandfish, not only in various potential sites in the Philippines, but also in many developing tropical countries in the Indo-Pacific.
    • Growth performance and condition factor of juvenile milkfish (Chanos chanos) cultured in a marine pen in relation to body size and temperature 

      Kodama, Masashi; Diamante, Rose Ann; Salayo, Nerissa D.; Castel, Raisa Joy G.; Sumbing, Joemel G. (Japan International Research Center for Agricultural Sciences (JIRCAS), 2021-04-01)
      The growth performance and condition factor (CF) of milkfish (Chanos chanos) in relation to body size and temperature were investigated in a shallow marine pen culture system in coastal waters of the Philippines. Three common models were tested to determine the best-fit model for the growth of milkfish, and the logistic model was found to be best for both weight-based and length-based growth. Daily specific growth rate in weight (\(DSGR_{W})\) and length (\(DSGR_{L})\) were positively correlated with water temperature, while both \(DSGR_{W}\) and \(DSGR_{L}\) were correlated negatively with the size of the fish, which supported the fit of the growth model. CF of milkfish exhibited a positive correlation with the feed conversion ratio and significant seasonal variations. The ratio was lower in the fast growth season (dry season) than in the slow growth season (rainy season). By integrating these controversial findings, we were able to posit that low temperature and inefficient feeding makes ‘fat’ fish and vice versa. This may be explained by changes in proximate body composition (e.g., protein and water content) and the change in metabolic rate brought about by different water temperatures.
    • Exploring, harnessing and conserving marine genetic resources towards a sustainable seaweed aquaculture 

      Brakel, Janina; Sibonga, Rema ORCID; Dumilag, Richard; Montalescot, Valeria; Campbell, Iona; Cottier-Cook, Elizabeth; Ward, Georgia; Le Masson, Virginie; Liu, Tao; Msuya, Flower; Brodie, Juliet; Lim, Phaik Eem; Gachon, Claire (Wiley Open Access, 2021-03-27)
      Seaweed cultivation is the fastest‐growing aquaculture sector, with a demonstrable potential to drive development in some of the poorest coastal populations worldwide. However, sustainable exploitation, fair access and equitable benefits from marine genetic resources, such as seaweeds have yet to be fully realised. Patchy fundamental knowledge on the genetic diversity and metabolic potential of algae limits their exploitation; scant practical skills and low investment in breeding restricts germplasm availability and the Nagoya protocol has only partially remediated insufficient governance. Further developments and the addressing of knowledge gaps in relation to biosecurity, breeders’ rights and conservation of genetic resources are needed for progress.
    • Biodiversity in mangrove-derived aquaculture ponds in Dumangas, Iloilo, Philippines 

      Bagarinao, Teodora ORCID (Science and Technology Information Institute, Department of Science and Technology, 2021-02)
      Large tracts of mangroves have been converted into aquaculture ponds and the consequent loss of biodiversity has been much decried. This paper shows that high aquatic biodiversity is retained in mangrove-derived aquaculture ponds in Dumangas, Iloilo, Philippines. Documentation of biodiversity was carried out over two years in 15 adjacent ponds (water areas 0.25–0.9 ha) used for farming penaeid shrimps, mud crabs, milkfish, seabass, rabbitfish, and other fishes. At harvest, these ponds yielded many extraneous non-crop or “bycatch” species, which had been naturally seeded by the tides and had grown (and some species reproduced) over the 2–10 mo that the ponds were underwater. From 21 crop cycles were obtained 8–56 species (from 6–30 taxonomic families) of bycatch fishes and macrocrustaceans with biomass of 2.5–168.4 kg ha–1 and abundance of 2–92 x 103 individuals ha–1, the differences due to pond area, duration of immersion, farming system, crop species, and the bycatch community. Some 85 species (44 families) of fishes occurred in the ponds, including 12 species of gobies, of which the small-size Acentrogobius viganensis, Pseudogobius javanicus, Mugilogobius cavifrons, and Gobiopterus panayensis reached high abundance. Macrocrustaceans consisted of 30 species (nine families), including several penaeid and palaemonid shrimps (Metapenaeus ensis and Nematopalaemon tenuipes were most common) and portunid and grapsid crabs. Mollusks in the ponds included 58 species (24 families), of which Cerithideopsilla cingulata was most abundant. The Dumangas ponds were leaky and allowed tidal recruitment of many mangrove animals despite net screens and chemical treatments. Existing tidal ponds serve as proxy mangrove lagoons (wetlands) that can harbor high aquatic biodiversity.
    • Production, feeding and storage of diatom Chaetoceros calcitrans paste by electrolytic flocculation with reduced Pb content 

      Villa-Franco, Annie; de la Peña, Milagros R.; Igcasan Jr., Hermoso P.; Gamuza, Glecilda B.; Teves, Christine Marie L.; Balinas, Vicente T. (Wiley, 2021-03-02)
      An innovative microalgae harvesting technique was carried out under various anode/cathode (2/2, 4/4 and 6/6) configuration and at different voltage treatment (3, 6 and 10 V) to efficiently electroflocculate Chaetoceros calcitrans. This study was conducted to determine the optimal configuration of electrode units (Pb and Al) and voltage settings that could improve the quality of harvested paste in terms of nutritional value and metal contamination for its later use in aquaculture. The use of 6/6 electrodes achieved the shortest harvest time of 48 ± 1 min and the lowest Pb content (83.7 ± 0.3 ppm). In terms of voltage treatment, harvest time was inversely proportional to the voltage used. However, Pb content was significantly lowest in the paste produced using 3 V (41.78 ± 0.12 ppm), when compared to those harvested under 6 V (304.35 ± 0.79 ppm) and 10 V (343.90 ± 0.91 ppm) treatment. Likewise, Pb residues in the resulting effluent are also lowest (0.245 ± 0 ppm) with 3 V treatments. C. calcitrans paste can be resuspended ≤6 months but with a lag phase of 3–4 days for use as starter culture. A 97% reduction in Pb content of C. calcitrans paste and 77% reduction in Pb content were obtained in Artemia (7.8%) fed C. calcitrans paste.
    • Changes in iodide and thyroid hormone levels of hatchery-reared orange-spotted rabbitfish Siganus guttatus (Bloch 1787) during early larval development 

      Cabanilla-Legaspi, Ma. Irene C.; Traifalgar, Rex Ferdinand; de Jesus-Ayson, Evelyn Grace; Andrino-Felarca, Karen Grace S.; Mamauag, Roger Edward ORCID (Elsevier, 2021-07)
      This paper elucidates the developmental profiles of iodide and thyroid hormones, thyroxine (T4) and triiodothyronine (T3) in hatchery-reared rabbitfish (Siganus guttatus) during early larval development. The study evaluated the levels of iodide, T4 and T3 tissue content in rabbitfish larvae at 0, 10, 20, 30, 40 and 50 days after hatching (DAH). Under normal rearing conditions, iodide (11.98 ± 4.3 nmol g−1) and thyroid hormones (T4: 0.09 ± 0.01 nmol g−1; T3: 0.01 ± 8.5E-05 nmol g−1 were already detected from newly-hatched larvae and their presence has been attributed from maternal origin. Iodide level sharply peaked at 10 DAH (1416.43 ± 149.6 nmol g−1) which is significantly higher compared to the iodide levels in the larvae at 20, 30, 40 and 50 DAH. The levels of THs gradually increased as the larvae developed with T4 (0.96 ± 0.05 nmol g−1) and T3 (0.03 ± 0.004 nmol g−1) exhibiting a peak on 20 and 30 DAH, respectively, which coincided with the onset of metamorphosis. Thyroid hormone levels gradually decreased which coincided also with the completion of metamorphosis. The present findings indicated a pattern of the rise and fall in iodide and thyroid hormone levels during larval development and its role in metamorphosis of rabbitfish larvae. The study has reported for the first time, the changes in iodide and thyroid hormone levels during the early developmental stage in hatchery-reared rabbitfish and the results are in agreement with those vital actions of thyroid hormones in other fish species.
    • The synergistic impacts of anthropogenic stressors and COVID-19 on aquaculture: A current global perspective 

      Sarà, Gianluca; Mangano, Maria Cristina; Berlino, Manuel; Corbari, Laura; Lucchese, Marta; Milisenda, Giacomo; Terzo, Stella Maria Concetta; Azaza, Mohamed Salah; Babarro, Jose; Bakiu, Rigers ORCID; Broitman, Bernardo; Buschmann, Alejandro; Christofoletti, Ronaldo; Deidun, Alan; Dong, Yunwei ORCID; Galdies, Johann; Glamuzina, Branko; Luthman, Ola; Makridis, Pavlos; Nogueira, António; Palomo, Maria Gabriela; Dineshram, Ramadoss; Rilov, Gil; Sanchez-Jerez, Pablo; Sevgili, Hüseyin; Troell, Max; AbouelFadl, Khaled; Md Adib, Mohamad Nor Azra; Britz, Peter; Brugere, Cecile; Carrington, Emily; Celić, Igor; Choi, Francis; Qin, Chuanxin; Dobroslavić, Tatjana; Galli, Paolo; Giannetto, Daniela; Grabowski, J.; Lebata-Ramos, Ma. Junemie Hazel ORCID; Lim, Po Teen; Liu, Yajie; Martínez-Llorens, Silvia; Maricchiolo, Giulia; Mirto, Simone; Pecarevic, Marijana; Ragg, Norman; Ravagnan, Elisa; Saidi, Djamal; Schultz, K.; Shaltout, Mohamed; solidoro, cosimo; Shau Hwai, Aileen Tan; Thiyagarajan , Vengatesen; Helmuth, Brian (Taylor and Francis, 2022-01-02)
      The rapid, global spread of COVID-19, and the measures intended to limit or slow its propagation, are having major impacts on diverse sectors of society. Notably, these impacts are occurring in the context of other anthropogenic-driven threats including global climate change. Both anthropogenic stressors and the COVID-19 pandemic represent significant economic challenges to aquaculture systems across the globe, threatening the supply chain of one of the most important sources of animal protein, with potential disproportionate impacts on vulnerable communities. A web survey was conducted in 47 countries in the midst of the COVID-19 pandemic to assess how aquaculture activities have been affected by the pandemic, and to explore how these impacts compare to those from climate change. A positive correlation between the effects of the two categories of drivers was detected, but analysis suggests that the pandemic and the anthropogenic stressors affect different parts of the supply chain. The immediate measurable reported losses varied with aquaculture typology (land vs. marine, and intensive vs. extensive). A comparably lower impact on farmers reporting the use of integrated multitrophic aquaculture (IMTA) methods suggests that IMTA might enhance resilience to multiple stressors by providing different market options under the COVID-19 pandemic. Results emphasize the importance of assessing detrimental effects of COVID-19 under a multiple stressor lens, focusing on areas that have already locally experienced economic loss due to anthropogenic stressors in the last decade. Holistic policies that simultaneously address other ongoing anthropogenic stressors, rather than focusing solely on the acute impacts of COVID-19, are needed to maximize the long-term resilience of the aquaculture sector.