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dc.contributor.authorAmar, Edgar C.
dc.contributor.authorSomera, Mechil D.
dc.contributor.authorMadero, Sherlyn B.
dc.contributor.authorTendencia, Eleonor A.
dc.contributor.authorFaisan Jr., Joseph P.
dc.contributor.editorQuinitio, Emilia T.
dc.contributor.editorParado-Estepa, Fe Dolores
dc.contributor.editorColoso, Relicardo M.
dc.date.accessioned2017-08-25T09:06:33Z
dc.date.accessioned2017-08-25T16:34:07Z
dc.date.available2017-08-25T09:06:33Z
dc.date.available2017-08-25T16:34:07Z
dc.date.issued2017
dc.identifier.citationAmar, E. C., Somera, M. D., Madero, S. B., Tendencia, E. A., & Faisan Jr., J. P. (2017). Strategies to reduce disease incidence in mud crab culture. In E. T. Quinitio, F. D. Parado-Estepa, & R. M. Coloso (Eds.), Philippines : In the forefront of the mud crab industry development : proceedings of the 1st National Mud Crab Congress, 16-18 November 2015, Iloilo City, Philippines (p. 154). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.en
dc.identifier.isbn9789719931072
dc.identifier.urihttp://hdl.handle.net/10862/3189
dc.descriptionAbstract only.en
dc.description.abstractMud crab hatchery suffers from low survival due to susceptibility to bacterial infection in the early larval stages among many causes. Despite food safety issues, antibiotics continue to be used in the absence of effective alternatives. In this study, screening of plant extracts was conducted to determine their suitability as antimicrobial agents against pathogens causing low survival in the hatchery. In addition, potential probionts were isolated from the gastrointestinal tract of mud crab, and water and sediments of mud crab grow-out ponds. Crude ethanolic extracts from various terrestrial and mangrove trees were tested for in vitro antimicrobial activity and toxicity to mud crab zoea and megalopa. In addition, the in vivo antimicrobial efficacies of the selected extracts were tested by oral administration followed by experimental challenge with Vibrio harveryi. On the other hand, the putative probionts, were tested for pathogenicity against mud crab zoea and megalopa and quorum sensing inhibition activity against V. harveyi. Finally the extracts and probionts were tested for their efficacy in simulated hatchery and grow-out trials. Results showed that extracts of Terminalia cattapa and the potential probiont Bacillus subtilis G100R11 showed antimicrobial and probiotic activity in in vitro and in vivo tests. In simulated hatchery trials, T. cattapa administration successfully produced crab instar with a survival of 1.3-1.8% in trials 1 and 2 comparable to antibiotic control. B. subtilis produced crab instar with survival of 0.8-1.0% in trials 1 and 2, better than the commercial probiotic with 0-0.13% survival. Using T. cattapa and B. subtilis, survival was above 30% until zoea 5 but suddenly dropped below 5% during metamorphosis to megalopa where high incidence of incomplete molting was observed. If difficulty affecting the molting process is addressed, high survival from zoea to megalopa and crab instar will be achievable.en
dc.language.isoenen
dc.publisherAquaculture Department, Southeast Asian Fisheries Development Centeren
dc.subjectScylla
dc.subjectTerminalia cattapa
dc.subjectVibrio harveryi
dc.subjectPhilippinesen
dc.titleStrategies to reduce disease incidence in mud crab cultureen
dc.typeConference paperen
dc.citation.spage154
dc.citation.conferenceTitlePhilippines : In the forefront of the mud crab industry development : proceedings of the 1st National Mud Crab Congress, 16-18 November 2015, Iloilo City, Philippinesen
dc.subject.asfaAntibioticsen
dc.subject.asfaBacterial diseasesen
dc.subject.asfaBioactive compoundsen
dc.subject.asfaCrab cultureen
dc.subject.asfaDevelopmental stagesen
dc.subject.asfaDisease controlen
dc.subject.asfaGrowing pondsen
dc.subject.asfaHatcheriesen
dc.subject.asfaHusbandry diseasesen
dc.subject.asfaLarvaeen
dc.subject.asfaMarine crustaceansen
dc.subject.asfaMetamorphosisen
dc.subject.asfaMortalityen
dc.subject.asfaMoultingen
dc.subject.asfaPond cultureen
dc.subject.asfaProbioticsen
dc.subject.asfaProphylaxisen
dc.subject.asfaSurvivalen
dc.subject.asfaTherapyen
dc.subject.asfaZoeaeen


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