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Biomass and reproductive states of Gracilaria heteroclada Zhang et Xia collected from Jaro, central Philippines
(Walter de Gruyter, 1996)
Biomass production and reproductive states were investigated to determine the abundance of Gracilaria heteroclada Zhang et Xia at Jaro, central Philippines from March 1990 to February 1991. Biomass of Gracilaria heteroclada ...
Effect of growth hormone and γ-aminobutyric acid on Brachionus plicatilis (Rotifera) reproduction at low food or high ammonia levels
(Elsevier, 1999)
Growth hormone (GH, 0.0025 and 0.025 I.U. ml<sup>−1</sup>) and γ-aminobutyric acid (GABA, 50 μg ml<sup>−1</sup>) enhance rotifer population growth in batch cultures. In order to further understand the mechanism of their ...
Effect of juvenile hormone and serotonin (5-HT) on mixis induction of the rotifer Brachionus plicatilis Muller
(Elsevier, 2000)
Juvenile hormone (JH) and serotonin (5-HT) were previously shown to enhance mictic (sexual) female production of the rotifer Brachionus plicatilis in batch cultures. To explore the basis of these effects, experiments were ...
Testicular histology and serum steroid hormone profiles in hatchery-bred catfish Clarias macrocephalus (Gunther) during an annual reproductive cycle
(Japanese Society of Fisheries Science, 1997)
Testicular development, gonadosomatic index (GSI), and related steroid hormones (testosterone or T, 11-ketotestosterone or 11-KT, 17α, 20β-dihydroxy-4-pregnen-3-one or DHP) in serum were monitored during an annual reproductive ...
Ovarian development and changes in the serum vitellogenin levels in the river sculpin, Cottus hangiongensis, during an annual reproductive cycle
(Faculty of Fisheries, Hokkaido University, 1989)
Annual changes in the ovarian development and serum vitellogenin concentration were investigated in the river sculpin, Cottus hangiongensis, sampled monthly from a river in southern Hokkaido, Japan. Ovarian development ...
Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper
(Elsevier, 2020-07-13)
A recombinant giant grouper Luteinizing Hormone (LH) consisting of tethered beta and alpha subunits was produced in a yeast expression system. The giant grouper LH β-subunit was also produced and administered to rabbits ...
Development of specific enzyme-linked immunosorbent assay for yellowtail kingfish (Seriola lalandi) follicle stimulating hormone using recombinant gonadotropins
(Elsevier, 2019)
We developed a specific competitive enzyme-linked immunosorbent assay (ELISA) for yellowtail kingfish (Seriola lalandi) follicle stimulating hormone (FSH). We previously produced a full-length single chain recombinant ...
Reproductive performance, lipids and fatty acids of mud crab Scylla serrata (Forsskål) fed dietary lipid levels
(Blackwell Publishing, 2007)
Natural food (NF, control), artificial diets (AD) containing total lipid levels of 10%, 12% and 14% (AD10, AD12 and AD14) and their combinations (AD10+NF, AD12+NF and AD14+NF) were fed for 112 days to pond-sourced ...
Production of microalgal paste in the Philippines
(Philippine Science Letters, 2020)
Technical advances have made possible the production of microalgal concentrate in paste form. Chaetoceros calcitrans was concentrated and made into an algal paste through centrifugation, chemical flocculation, and electrolytic ...
Reproduction, early development, and larval rearing of the climbing perch Anabas testudineus (Teleostei: Anabantidae) in captivity
(College of Agriculture and Food Science, University of the Philippines Los Baños, 2023-06)
Induced spawning, early development, and larval rearing of the climbing perch Anabas testudineus were examined under captive conditions. Five female climbing perch [125.33 ± 3.27 mm standard length (SL); 70.67 ± 5.59 g body weight (BW)] were each paired with apparently mature males (108.50 ± 3.97 mm SL; 39.27 ± 4.70 g BW) and induced to spawn with 5,000 IU human chorionic gonadotropin (hCG) + 0.5 mL Ovaprim/kg BW. All hCG + Ovaprim-injected fish spawned 9 – 11 h after hormone administration but saline-injected fish (control group) failed to spawn. Mean egg production per female, fertilization and hatching rates, and larval production per female were 7,667 ± 1,313, 98.11 ± 1.63%, 61.01 ± 19.92%, and 4,435 ± 1,041, respectively. Fertilized eggs (1.52 – 1.96 mm in diameter) were almost spherical, non-adhesive, transparent, and buoyant due to a large, single oil globule (515 ± 33 µm in diameter). First cleavage appeared 30 min post-fertilization (MPF) and egg development lasted for 20 – 24 h post-fertilization (HPF) (29°C). Newly hatched climbing perch larvae [1.73 ± 0.08 mm in total length (TL)] with yolk volume (YV) and oil globule volume (OGV) of 0.545 ± 0.104 mm<sup>3</sup> and 0.072 ± 0.013 mm<sup>3</sup>, respectively had no pigmented eyes or functional mouth parts and digestive tract, and immobile with the yolk sac pointed upward. Eyes became pigmented and both mouth and anus opened in 1 d old larvae (2.09 ± 0.04 mm TL) in preparation for exogenous feeding. Except for body depth (BD) and pre-anal length (PAL), a general increase in TL (1.73 – 3.72 mm), eye diameter (ED) (0.164 – 0.378 mm), head length (HL) (0.205 – 0.923 mm), and mouth gape (MG) size (0.308 – 0.552 mm) was observed, coinciding with yolk resorption in 7 d post-hatched larvae. Climbing perch larvae were reared at four stocking densities (25, 50, 75, 100 larvae L-1) and fed solely on live food (Brachionus rotundiformis from days 2 to 12 and Artemia nauplii from days 13 to 40) or co-fed live food and microparticulate diet (MPD) (Artemia nauplii from days 2 to 15 and Artemia + MPD from days 16 to 40). Mean survival rates at lower densities (25 – 50 larvae L<sup>-1</sup>) were significantly higher than those reared at 75 and 100 larvae L<sup>-1</sup> (P < 0.05). Furthermore, climbing perch larvae co-fed Artemia nauplii and MPD showed better survival (28.67 – 81.00%) than those fed solely on live food (24.88 – 64.00%). However, at the end of the 40 d trial period, growth parameters did not vary significantly among densities (P > 0.05), except for the condition factor in larvae co-fed Artemia nauplii and MPD. Taken together, results demonstrate the effectiveness of hCG + Ovaprim for captive breeding and observations on the early development of climbing perch, and the feasibility of rearing the larvae at 25 – 50 L<sup>-1</sup> on a combination of Artemia nauplii and MPD under laboratory conditions....