Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff: Recent submissions
541-560 / 1693
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WSSV risk factors related to water physico-chemical properties and microflora in semi-intensive Penaeus monodon culture ponds in the Philippines
(Elsevier, 2010)Whitespot syndrome virus, WSSV, is the most important among the shrimp diseases. One of the suggested WSSV risk factors is the occurrence of stress since stressors could compromise the shrimp defence system thus increasing the risk of WSSV infection. Stressors are usually related to the physico-chemical properties of both water and pond bottom. This paper investigates the effect of some biotic and abiotic components of the pond ecosystem on WSSV infection and outbreak. Water physico-chemical properties and microflora of 91 production cycles of 8 semi-intensive shrimp farms were analyzed to determine WSSV risk factors, using factor analysis and logistic regression. Fluctuations of temperature and pH are important risk factors that will result to an infection but not necessarily to an outbreak. Exposure to high salinity and high temperature are important factors for an infection to result to an outbreak. The risk of an infection is reduced when the water temperature is high, salinity fluctuations are small, and percentage of yellow Vibrio colonies is greater than the green ones. Further studies are needed to clarify the effects of water depth, water transparency, and various bacterial counts; these factors could be individual or interactive. -
Effect of different sizes of saline red tilapia hybrid Oreochromis niloticus Linnaeus x O. mossambicus Peters on the growth of luminous bacteria Vibrio harveyi
(College of Agriculture, University of the Philippines Los Baños, 2010)Luminous bacterial disease caused by Vibrio harveyi has devasted the shrimp industry. The use of different strains of tilapia and other fish species polycultured with shrimp cultured at a salinity of 24 ppt to control luminous bacteria has been reported. These species, however, could not tolerate salinities higher than 24 ppt. Alternative species/strains that could be used to control luminous bacteria at high salinities need to be investigated. Likewise, the effect of fish size on the growth of luminous bacteria is worth studying. Shrimp (Penaeus monodon Fabricius) were stocked in concrete tanks filled with 34 ppt cartridge filtered seawater. Tanks were stocked with two sizes (16 pcs, average body weight (ABW) = 55 g and 6 pcs ABW=250 g) of the red tilapia hybrid Oreochromis mossambicus Peters x O. niloticus Linnaeus, except for the tanks that served as the control. Luminous bacteria (V. harveyi) were inoculated into the tank water to a density of 104 Cfu mL-1. Significantly lower luminous bacterial count was observed in tanks with either of the two sizes of red tilapia hybrid compared with the control, after 5-7 d. Bacteria isolated from the fish feces, mucus and rearing water; and the fish feces and mucus themselves demonstrated anti-V. harveyi activity. Results showed that the red tilapia hybrid could be used to control luminous bacteria at salinities as high as 34 ppt and that the size of the fish did not affect its efficiency. The ability of the red tilapia hybrid to control the growth of the luminous bacteria V. harveyi is attributed to different factors such as the mucus, the feces and the bacteria associated with tilapia culture. Bacteria associated with the fish mucus and feces also contributed to the anti-V. harveyi activity of these biological substances. -
Koi herpesvirus-associated mortalities in quarantined koi carp in the Philippines
(European Association of Fish Pathologists, 2010)Illegally imported koi carp were confiscated at the Ninoy Aquino International Airport (NAIA), Manila, Philippines by the Fisheries Quarantine and Inspection Service Officers of the Bureau of Fisheries and Aquatic Resources (BFAR). The confiscated fish were turned over to the BFAR Fish Health Laboratory where they were held for observation at a water temperature of 28 degree C. After 5 days, some fish were showing abnormal swimming behavior and some had died. The most prominent disease signs in the freshly dead and moribund fish were body ulcerations and pale gills showing white necrotic patches, consistent with the clinical signs of KHV infection. Gills were dissected and fixed in 95% ethanol. All of the samples tested positive for KHV in a 1-step PCR assay. This paper reports the first case of KHV associated mortalities in illegally important koi carp confiscated by the Fisheries Quarantine and Inspection Service Officers of BFAR. This highlights the importance of the quarantine and inspection service s role in preventing the illegal entry of fish into the country and the introduction of exotic aquatic diseases. -
Growth comparison of Asian Nile and red tilapia strains in controlled and uncontrolled farm conditions
(European Aquaculture Society, 2010)Growth of several genetically improved Nile tilapia (GIFT or Genetically Improved Farmed Tilapia, FaST or Freshwater Aquaculture Center Selected Tilapia, SEAFDEC-selected) and domesticated red tilapia (BFS or Binangonan Freshwater Station, NIFI-red or National Inland Fisheries Institute red, HL or Hacienda Luisita) stocks were compared in controlled (tank) and uncontrolled farm conditions (lake-based cages) with unselected NIFI or Chitralada Nile tilapia as control. Specific growth rate differed significantly (P = 0.009) in tank-reared Nile tilapia stocks where GIFT grew best at 1.358%/day followed by FaST (1.307%/day), control stock NIFI (1.257%/day) and SEAFDEC-selected (1.202%/day). Genetic effect explained 84.4% of the variance in growth of Nile tilapia in tanks. Although Nile tilapia growth in cages followed the same trend where GIFT grew best at 1.570%/day, no significant stock differences (P = 0.479) were noted. Meanwhile, red tilapia reared in either tanks or cages showed no significant stock differences in terms of growth. However, survival of the red tilapia stocks in cages differed significantly with HL having the highest percentage survival at 93.3%. The different growth responses of the Nile tilapia stocks especially under controlled (tank) farm conditions were largely due to genetic factors (stock differences).Under uncontrolled farm conditions, environmental factors were generally observed to have also affected the survival and to some extent, the growth of Asian Nile and red tilapia stocks. -
Protective immunity against viral nervous necrosis (VNN) in brown-marbled grouper (Epinephelus fuscogutattus) following vaccination with inactivated betanodavirus
(Academic Press, 2010)Viral nervous necrosis (VNN) caused by betanodaviruses has been recently implicated in serious mortalities of groupers in the grow-out culture system. A safe and effective vaccine against this disease is urgently needed. This study demonstrates that a single intramuscular vaccination with formalin-inactivated Philippine strain of piscine betanodavirus (genotype: redspotted grouper nervous necrosis virus; RGNNV) induces potent immune responses and substantial protective immunity against an intramuscular challenge with the homologous virus in brown-marbled grouper, Epinephelus fuscogutattus, a highly susceptible marine fish species to VNN. Seroneutralization assay conducted on sera of vaccinated fish revealed the occurrence of substantial neutralizing-antibody titers from Days 15 (mean titer 1:800) to 190 (1:400) with the highest titer observed at Day 60 post-vaccination (1:5120). When vaccinated fish were challenged with the homologous virus at Days 15, 30 and 75 post-vaccination, significantly higher survival rates were obtained in these fish compared with their corresponding controls (L-15 injected fish). Abrogation of virus multiplication in all vaccinated survivors was indicated by undetectable virus titers in the brains and kidneys paralleled by significantly high levels of neutralizing antibodies in the sera of these fish. Consecutively, replicates of vaccinated fish that survived betanodavirus challenge at Days 15 and 75 post-vaccination were maintained in flow-through aquaria and rechallenged with the homologous virus 3 and 5 months later, respectively. A significant drop in neutralizing-antibody titers of 3 and 8 folds, respectively, were observed in the sera of Days 15 and 75 post-vaccinated fish assayed before the virus rechallenge. Interestingly, reversion in the levels of neutralizing antibodies to significantly high levels (8–15 folds) were noted in these fish after the virus rechallenge. Taken together, our current data clearly demonstrate that a single administration of the inactivated Philippine strain of betanodavirus vaccine can effectively mount a specific anamnestic response and concomitant long-term protection against VNN in grouper at the grow-out culture system. -
Growing the reproductive cells (carpospores) of the seaweed, Kappaphycus striatum, in the laboratory until outplanting in the field and maturation to tetrasporophyte
(Springer Verlag, 2010)Carposporophytes of the seaweed, Kappaphycus striatum, from the wild were made to shed spores in the laboratory and grown in multi-step culture method until they reached maturity. For each succeeding transfer onto increasingly bigger culture vessels, there was a marked increase in the growth of carposporelings. When plantlets were ready for outdoor culture, they were placed in aquaria and concrete tanks and later moved to the sea in net cage and long-line for grow-out culture. Successfully growing sporelings from carposporophytes in the laboratory until they reach market size seems to depend on the stage of sporelings and environmental factors such as photoperiod and temperature. In this study, carpospore progenies (diploids) also matured into tetrasporophytes and haploid progenies showed resistance to higher temperature. -
Growth and survival of hatchery-bred giant clams (Tridacna gigas) in an ocean nursery in Sagay Marine Reserve, Philippines
(European Aquaculture Society, 2010)To restore the diminishing population of the giant clam Tridacna gigas in Sagay Marine Reserve (SMR), Negros Occidental, central Philippines, two size classes [8- and 10-cm shell length (SL)] of hatchery-bred T. gigas were reared in an adjacent ocean nursery for restocking to Carbin Reef later upon reaching grow-out size of ≥20 cm SL. Growth rates did not significantly differ for both sizes and were on average 0.67 cm month−1. However, survival after 382 days of rearing T. gigas was significantly higher in the 10-cm SL clams than the 8-cm SL clams (96 and 83%, respectively). For future restocking projects, the use of 8-cm SL clams is recommended because the lower survival of this size class is compensated by its cheaper price. While rearing the clams to attain grow-out size, the population of wild clams (Family Tridacnidae) in Carbin Reef was assessed using ten 50 × 2-m belt transects. Four species of tridacnid clams have been recorded: Hippopus hippopus, Tridacna crocea, T. maxima>, and T. squamosa. T. crocea comprised 12.5–93.9% of all the clams observed in all ten transects. There was a significant difference in clam density between species (ANOVA, F = 6.94, P < 0.001), with T. crocea having the highest density. Living T. gigas were absent, but presence of dead shells was indicative of its presence in the reef in the past. It can be expected that the release of hatchery-bred T. gigas juveniles in Carbin Reef could provide future breeders that will repopulate this reef and the adjacent reef communities. -
Effects of dietary l-tryptophan on the agonistic behavior, growth and survival of juvenile mud crab Scylla serrata
(Elsevier, 2010)The reduction of the survival of mud crab during culture has been largely attributed to aggressive encounters and cannibalism. In some crustaceans, suppressed aggression is linked to increased concentration of circulating and brain serotonin (5-hydroxytryptamine, 5-HT). Likewise, tryptophan (TRP), a precursor of 5-HT is reported to suppress the aggression and improve the survival of some cultured fish through dietary supplementation. We investigated the effects of feeding formulated diet with different TRP levels (0.32% as control, 0.5%, 0.75% and 1% of dry diet) on the agonistic behavior, growth and survival of juvenile mud crab Scylla serrata. Mud crabs were individually stocked and fed the experimental diets for 4 weeks before they were set to a one hour fight experiment. The fights were recorded using a video camera and the aggressiveness of the crabs was quantified. Hemolymph was sampled after 15 and 30 days of feeding (resting) and right after the fight to measure circulating 5-HT concentration. Higher TRP levels suppressed the aggressiveness of mud crab in a dose dependent manner. The intensity and frequency of attacks were both significantly lower (P < 0.05) in those given diets containing 0.75% and 1% TRP as compared with the control. Serotonin-ELISA assay revealed that 5-HT levels in the hemolymph before the fight (after 15 and 30 days; resting) were not significantly different between treatments. However after the fight, 5-HT concentration was significantly higher in TRP-supplemented mud crabs as compared with the control (0.5% = P < 0.05; 0.75% and 1% = P < 0.01). Furthermore, mud crabs (0.16 g BW) were reared in 0.40 m2 circular tanks at 20 crabs/tank and fed the experimental diets for 30 days to determine growth and survival. Survival was higher in TRP-supplemented mud crabs (0.5% = 35%, 0.75% = 33.33%, 1% = 35%) as compared with the control (18.33%). However, daily growth gain (DRG), relative growth rate (RGR) and specific growth rate (SGR) were reduced in TRP-supplemented groups than with the control group. In conclusion, the data shows that the aggressive behavior of juvenile mud crab can be suppressed by supplementation of L-TRP. The survival of juvenile mud crab can be improved by increasing the level of TRP to 0.5%–1%. However, higher TRP levels may affect growth of mud crab. TRP supplementation resulted to a significant increase of 5-HT concentration in the hemolymph which was clearly observed after the fight suggesting that 5-HT plays an important role in suppressing the agonistic behavior of mud crab during aggressive encounters. -
Cloning of IGF-I, IGF-II and IGF-IR cDNAs in mullet (Mugil cephalus) and grouper (Epinephelus coioides): Molecular markers for egg quality in marine fish
(Knowledgia Scientific, 2010)The putative cDNA sequence of insulin like growth factor (IGF)-I, IGF-II and IGF-IR (receptor) was determined from the tissues of mullet, Mugil cephalus and grouper Epinephelus coioides. The deduced partial coding sequences were deposited at Gene Bank (Accession No. AY427954, AY427955, AY772254, AY776158, AY776159, AY772255). These basic data were used to determine messenger ribonucleic acid (mRNA) levels during embryogenesis in the two species. Results showed that IGF-II has the highest expression ratio and makes it a potential marker for egg quality in the two cultured species. -
Settlement, growth and survival of donkey's ear abalone Haliotis asinina (Linne) in response to diatom diets and attachment substrate
(Science and Technology Information Institute, 2010)The effect of feeding four diatom diets (Amphora sp., Navicula ramosissima, Amphora sp. + N. ramosissima, and mixed diatoms) and two attachment substrates (PP+CCA: polyvinyl plates with crustose coralline algae; PP-CCA: polyvinyl plates without crustose coralline algae) were determined for seed production of abalone, Haliois asinina. On day 5, significantly higher number of larvae settled on PP+CCA fed with mixed diatoms followed by Amphora sp., N. ramosissima, Amphora sp. + N. ramosissima, and abalone larvae reared on PP-CCA fied with N. ramosissima only. Fewer larvae settled on PP-CCA fed with Amphora + N. ramosissima, Amphora sp. and mixed diatoms. The size of abalone juveniles from PP-CCA was significantly bigger compared with juveniles measured from PP+CCA. However, the number of juveniles harvested from tanks provided with PP+CCA was higher compared with tanks with PP-CCA. This study has shown that crustose coralline algae favored the settlement of H. asinina larvae and inoculation of diatom slurry is necessary to provide sufficient food for the growing larvae. -
Initial assessment of the bacterial population of Guimaras waters and soil after the Solar I oil spill
(University of the Philippines Visayas, 2009)A massive oil spill occurred near the shores of Guimaras Island, Philippines in 11 Aug 2006 caused by the Sunken MT Solar I vessel. The oil spill spread to neighboring towns of Guimaras damaging the marine coastal environment, consequently,causing health and economic problems, particularly, by affecting local fisheries, wildlife and tourism. Hence, this study was conducted to assess, quantify and isolate indigenous bacteria with potential petroleum hydrocarbon-degrading properties that could be used for bioremediation of oil spill contaminated areas in Guimaras and nearby provinces. -
Post-August 2006 oil spill populations of Penaeid shrimp in island and riverine mangroves in Guimaras, central Philippines
(University of the Philippines Visayas, 2009)To evaluate the impact on biota of the release of arrow up million liters of bunker oil off Guimaras Island, Central Philippines, the penaeid shrimp populations in a protected island mangrove (Tandog) and a riverine mangrove (Sibunag) were surveyed by pocket seine 2-3 months after the August 2006 spill; results are compared to 1993 baseline data. A total of 529 individuals belonging to three penaeid species were collected - Metapenaeus anchistus in Tandog, and Metapenaeus ensis and Penaeus merguiensis in Sibunag. Shrimp densities were significantly higher in Tandog Island in 2006 compared to 1993 but remained similar in the riverine mangrove. This suggests that protection may have a greater impact than the Oil Spill per se because Tandog is part of the protected Taklong National Marine Reserve. In contrast to abundance, shrimp sizes (carapace length) were significantly smaller in 2006 compared to 1993 for both sites. -
Immune responses of Asian sea bass, Lates calcarifer Bloch, against an inactivated betanodavirus vaccine
(Blackwell Publishing, 2009)Asian sea bass, Lates calcarifer (Bloch), exhibited strong immune responses against a single injection of the formalin-inactivated red-spotted grouper nervous necrosis virus (RGNNV), a betanodavirus originally isolated in Japan. Fish produced neutralizing antibodies at high titre levels from days 10 (mean titre 1:480) to 116 (1:1280), with the highest titre at day 60 post-vaccination (1:4480). When fish were challenged with the homologous RGNNV at day 54 post-vaccination, there were no mortalities in both the vaccinated and unvaccinated control fish. However, a rapid clearance of the virus was observed in the brains and kidneys of vaccinated fish, followed by a significant increase in neutralizing-antibody titres. Furthermore, the vaccine-induced antibodies potently neutralized Philippine betanodavirus isolates (RGNNV) in a cross-neutralization assay. The present results indicate the potential of the formalin-inactivated RGNNV vaccine against viral nervous necrosis (VNN) of Asian seabass. -
Managing fisheries conflicts through communication planning: Experience from inland fisheries of Bangladesh
(Elsevier, 2009)Increasing population, ineffective management, competition among fishing gears over access to resources and proliferation of destructive practices are imposing severe stress on the inland water bodies of Bangladesh. These factors also contribute to the increasing incidence of conflicts among fishery stakeholders. When unabated, these conflicts are potential threats to the livelihoods of millions of the poorest fishing communities that depend on these resources. Effective communication between conflicting parties is perceived as a key for establishing successful negotiations for managing conflicts. On this premise, this paper presents and assesses a Fisheries Conflicts Communication Framework, henceforth called FishCom, a tool for developing plans and strategies for managing fisheries conflicts in the inland fisheries of Bangladesh. This tool embodies a structured participatory process intended for use by policy-makers and conflict management practitioners. They have important roles in catalyzing and effecting changes that are instrumental in minimizing, if not totally eliminating conflicts. Experiences from applying FishCom in the inland fisheries study sites in Bangladesh show that it has enabled a systematic stakeholder-inclusive identification and evaluation of fisheries conflicts and planning of communication interventions to manage them. -
Surveillance of emerging fish viral pathogens in some Southeast Asian countries
(Society of Israeli Aquaculture and Marine Biotechnology, 2009)Preventing the transboundary movement of fish viral pathogens in a global environment requires active surveillance. This study examined the presence of three emerging viral pathogens among koi, common, grass, and silver carp in Cambodia, Lao PDR, Myanmar, Philippines, and Vietnam. The studied viruses included koi herpesvirus (KHV), spring viremia of carp virus (SVCV), and grass carp reovirus (GCRV). Detection methods consisted of virus isolation by cell culture, infection assay in naive fish, polymerase chain reaction (PCR), and reverse-transcriptase PCR (RT-PCR). Tissues were collected and pooled from 193 fish samples in Dec. 2004 to Feb. 2005, 406 in Sep. 2005 to Feb. 2006, and 1302 in Oct. 2006 to Feb. 2007. For cell culture, tissue filtrates were prepared from pooled spleens, kidneys, livers, and gills and inoculated onto koi fin (KF-1), grass carp kidney (GCK), and fat head minnow (FHM) cells. For infection assay, tissue filtrates were injected intraperitoneally to healthy, naive common carp. No virus was detected after three cell culture passages and the infection bioassays. One-step and nested-step PCR was used to detect KHV in gills of fish samples. One-step and semi-nested RT-PCR was used to detect SVCV and GCRV in the spleens, kidneys, and livers of fish samples. Samples from all three years from all five countries yielded negative results for all three viruses, indicating that KHV, SVCV, and GCRV were not present in these five countries during the period of the study although KHV outbreaks had been detected in Indonesia, Taiwan, Japan, Thailand, China, and Malaysia. -
Evaluation of hatchery-based enhancement of the mud crab, Scylla spp., fisheries in mangroves: comparison of species and release strategies
(CSIRO Publishing, 2009)Ranching, stock enhancement and restocking are management approaches involving the release of wild or hatchery-bred organisms to enhance, conserve or restore fisheries. The present study, conducted from April 2002 to November 2005, evaluated the effectiveness of releasing wild and hatchery-reared (HR) mud crabs in the mangroves of Ibajay, Aklan, Philippines where preliminary studies demonstrated declining fishery yields, abundance and size of crabs. Comparison of survival and growth of wild-released and HR Scylla olivacea and HR Scylla serrata demonstrated the effect of nursery conditioning, size-at-release and species differences. Overall yield and catch per unit effort (CPUE) increased by 46% after stock enhancement trials. Recapture rates of released crabs were highest in wild-released S. olivacea and in crabs measuring 65.0–69.9 mm carapace width (CW) and lowest in non-conditioned HR S. serrata. Growth rates were highest for conditioned HR S. olivacea and lowest for conditioned HR S. serrata (11.7 and 3.7 mm month-1 respectively). Fishing mortality was highest for S. olivacea, whereas natural mortality was greater for S. serrata. Conditioning hatchery-bred animals before release is also important in obtaining higher survival. S. olivacea was the more appropriate of the two species for release in mangrove habitats inundated with low-salinity water. However, there is a need for site-specific studies to evaluate the effectiveness of releases. -
Molecular epidemiology of koi herpesvirus
(Japanese Society of Fish Pathology, 2009)Three regions of koi herpesvirus (KHV) genomic DNA were compared for 34 samples from Japan, six from Indonesia, two from Taiwan, one from the Philippines, 13 from the Netherlands, one from the UK, one from the USA and one from Israel. The analyzed genomic regions included known PCR-detection targets (SphI-5, 9/5 and the thymidine kinase gene). The KHVs from Asian countries were very homogeneous, although two variants were noted based on a single nucleotide polymorphism. In contrast, seven variants were found in KHVs from outside of Asia, and although closely related to one another, they were clearly distinct from those from Asian. The results suggest that a clear genetic distinction exists between Asian and European (including each single isolate from the USA and Israel) types of KHV, and that unique types of KHV were independently introduced or emerged in the respective geographic locations. -
Non-linearity in ecosystem services: temporal and spatial variability in coastal protection
(Ecological Society of America, 2009)Natural processes tend to vary over time and space, as well as between species. The ecosystem services these natural processes provide are therefore also highly variable. It is often assumed that ecosystem services are provided linearly (unvaryingly, at a steady rate), but natural processes are characterized by thresholds and limiting functions. In this paper, we describe the variability observed in wave attenuation provided by marshes, mangroves, seagrasses, and coral reefs and therefore also in coastal protection. We calculate the economic consequences of assuming coastal protection to be linear. We suggest that, in order to refine ecosystem-based management practices, it is essential that natural variability and cumulative effects be considered in the valuation of ecosystem services. -
Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties
(Springer Verlag, 2009)Three varieties of Kappaphycus alvarezii (Kapilaran, KAP), Tambalang purple (PUR), Adik-adik (AA), and one variety of Kappaphycus striatum var. sacol (green sacol (GS) were used to determine the efficiency of Acadian marine plant extract powder (AMPEP) as a culture medium at different concentrations, for the regeneration of young plants of Kappaphycus varieties, using tissue culture techniques for the production of seed stock for nursery and outplanting purposes for the commercial cultivation of carrageenophytes. A shorter duration for shoot formation was observed when the explant was treated with AMPEP + Plant Growth Regulator (PGR = PAA + zeatin at 1 mg L−1) compared to AMPEP when used singly. However, four explants responded differently to the number of days required for shoot formation. The KAP variety took 46 days to form shoots at 3–4 mg L−1 AMPEP + PGR; while PUR required 21 days at 3–5 mg L−1 AMPEP and 3–4 mg L−1 AMPEP + PGR. AA required 17 days at 3–5 mg L−1 AMPEP and AMPEP + PGR; and GS 25 days at 1 mg L−1 AMPEP + PGR. It was observed that among the four explants used, PUR and AA initiated shoot formation with the use of AMPEP only at higher concentrations (3–5 mg L−1) after a shorter period. Only PUR responded positively to ESS/2 for shoot initiation. The use of AMPEP alone and/or in combination with PGR as a culture medium in the propagation of microplantlets using tissue culture technique is highly encouraging. -
Price dynamics and cointegration in the major markets of aquaculture species in the Philippines
(Southeast Asian Regional Center for Graduate Study and Research in Agriculture, 2009)This study explored the interdependencies of aquaculture markets in the Philippines by establishing price cointegration between wholesale and retail prices of three major species commonly farmed in the country, i.e., milkfish, tilapia and shrimp. The co-movements of wholesale prices between and among key markets for each species were also investigated. Moreover, exogeneity in prices was established using Granger-causation model to determine the existence of price leaders among these markets. Such information are crucial in farm management decisions of producers and traders through better understanding of the efficiency in price formation across production and consumption centers which in turn defines the movements in prices and products among markets. Appropriate policies for the development of markets for the three aquaculture species were also identified. Such policies are expected to contribute towards the attainment of efficient pricing and distribution of benefits among market players and stakeholders. These benefits are expected to manifest through the system of grading standards for fish traded in local markets and in the choice of cost-effective technologies in grow-out and post-production practices.





















