SEAFDEC/AQD Institutional Repository: Recent submissions
Now showing items 2261-2280 of 4221
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Cloning of IGF-I, IGF-II and IGF-IR cDNAs in mullet (Mugil cephalus) and grouper (Epinephelus coioides): Molecular markers for egg quality in marine fish
(Knowledgia Scientific, 2010)The putative cDNA sequence of insulin like growth factor (IGF)-I, IGF-II and IGF-IR (receptor) was determined from the tissues of mullet, Mugil cephalus and grouper Epinephelus coioides. The deduced partial coding sequences were deposited at Gene Bank (Accession No. AY427954, AY427955, AY772254, AY776158, AY776159, AY772255). These basic data were used to determine messenger ribonucleic acid (mRNA) levels during embryogenesis in the two species. Results showed that IGF-II has the highest expression ratio and makes it a potential marker for egg quality in the two cultured species. -
Settlement, growth and survival of donkey's ear abalone Haliotis asinina (Linne) in response to diatom diets and attachment substrate
(Science and Technology Information Institute, 2010)The effect of feeding four diatom diets (Amphora sp., Navicula ramosissima, Amphora sp. + N. ramosissima, and mixed diatoms) and two attachment substrates (PP+CCA: polyvinyl plates with crustose coralline algae; PP-CCA: polyvinyl plates without crustose coralline algae) were determined for seed production of abalone, Haliois asinina. On day 5, significantly higher number of larvae settled on PP+CCA fed with mixed diatoms followed by Amphora sp., N. ramosissima, Amphora sp. + N. ramosissima, and abalone larvae reared on PP-CCA fied with N. ramosissima only. Fewer larvae settled on PP-CCA fed with Amphora + N. ramosissima, Amphora sp. and mixed diatoms. The size of abalone juveniles from PP-CCA was significantly bigger compared with juveniles measured from PP+CCA. However, the number of juveniles harvested from tanks provided with PP+CCA was higher compared with tanks with PP-CCA. This study has shown that crustose coralline algae favored the settlement of H. asinina larvae and inoculation of diatom slurry is necessary to provide sufficient food for the growing larvae. -
Initial assessment of the bacterial population of Guimaras waters and soil after the Solar I oil spill
(University of the Philippines Visayas, 2009)A massive oil spill occurred near the shores of Guimaras Island, Philippines in 11 Aug 2006 caused by the Sunken MT Solar I vessel. The oil spill spread to neighboring towns of Guimaras damaging the marine coastal environment, consequently,causing health and economic problems, particularly, by affecting local fisheries, wildlife and tourism. Hence, this study was conducted to assess, quantify and isolate indigenous bacteria with potential petroleum hydrocarbon-degrading properties that could be used for bioremediation of oil spill contaminated areas in Guimaras and nearby provinces. -
Post-August 2006 oil spill populations of Penaeid shrimp in island and riverine mangroves in Guimaras, central Philippines
(University of the Philippines Visayas, 2009)To evaluate the impact on biota of the release of arrow up million liters of bunker oil off Guimaras Island, Central Philippines, the penaeid shrimp populations in a protected island mangrove (Tandog) and a riverine mangrove (Sibunag) were surveyed by pocket seine 2-3 months after the August 2006 spill; results are compared to 1993 baseline data. A total of 529 individuals belonging to three penaeid species were collected - Metapenaeus anchistus in Tandog, and Metapenaeus ensis and Penaeus merguiensis in Sibunag. Shrimp densities were significantly higher in Tandog Island in 2006 compared to 1993 but remained similar in the riverine mangrove. This suggests that protection may have a greater impact than the Oil Spill per se because Tandog is part of the protected Taklong National Marine Reserve. In contrast to abundance, shrimp sizes (carapace length) were significantly smaller in 2006 compared to 1993 for both sites. -
Immune responses of Asian sea bass, Lates calcarifer Bloch, against an inactivated betanodavirus vaccine
(Blackwell Publishing, 2009)Asian sea bass, Lates calcarifer (Bloch), exhibited strong immune responses against a single injection of the formalin-inactivated red-spotted grouper nervous necrosis virus (RGNNV), a betanodavirus originally isolated in Japan. Fish produced neutralizing antibodies at high titre levels from days 10 (mean titre 1:480) to 116 (1:1280), with the highest titre at day 60 post-vaccination (1:4480). When fish were challenged with the homologous RGNNV at day 54 post-vaccination, there were no mortalities in both the vaccinated and unvaccinated control fish. However, a rapid clearance of the virus was observed in the brains and kidneys of vaccinated fish, followed by a significant increase in neutralizing-antibody titres. Furthermore, the vaccine-induced antibodies potently neutralized Philippine betanodavirus isolates (RGNNV) in a cross-neutralization assay. The present results indicate the potential of the formalin-inactivated RGNNV vaccine against viral nervous necrosis (VNN) of Asian seabass. -
Managing fisheries conflicts through communication planning: Experience from inland fisheries of Bangladesh
(Elsevier, 2009)Increasing population, ineffective management, competition among fishing gears over access to resources and proliferation of destructive practices are imposing severe stress on the inland water bodies of Bangladesh. These factors also contribute to the increasing incidence of conflicts among fishery stakeholders. When unabated, these conflicts are potential threats to the livelihoods of millions of the poorest fishing communities that depend on these resources. Effective communication between conflicting parties is perceived as a key for establishing successful negotiations for managing conflicts. On this premise, this paper presents and assesses a Fisheries Conflicts Communication Framework, henceforth called FishCom, a tool for developing plans and strategies for managing fisheries conflicts in the inland fisheries of Bangladesh. This tool embodies a structured participatory process intended for use by policy-makers and conflict management practitioners. They have important roles in catalyzing and effecting changes that are instrumental in minimizing, if not totally eliminating conflicts. Experiences from applying FishCom in the inland fisheries study sites in Bangladesh show that it has enabled a systematic stakeholder-inclusive identification and evaluation of fisheries conflicts and planning of communication interventions to manage them. -
Surveillance of emerging fish viral pathogens in some Southeast Asian countries
(Society of Israeli Aquaculture and Marine Biotechnology, 2009)Preventing the transboundary movement of fish viral pathogens in a global environment requires active surveillance. This study examined the presence of three emerging viral pathogens among koi, common, grass, and silver carp in Cambodia, Lao PDR, Myanmar, Philippines, and Vietnam. The studied viruses included koi herpesvirus (KHV), spring viremia of carp virus (SVCV), and grass carp reovirus (GCRV). Detection methods consisted of virus isolation by cell culture, infection assay in naive fish, polymerase chain reaction (PCR), and reverse-transcriptase PCR (RT-PCR). Tissues were collected and pooled from 193 fish samples in Dec. 2004 to Feb. 2005, 406 in Sep. 2005 to Feb. 2006, and 1302 in Oct. 2006 to Feb. 2007. For cell culture, tissue filtrates were prepared from pooled spleens, kidneys, livers, and gills and inoculated onto koi fin (KF-1), grass carp kidney (GCK), and fat head minnow (FHM) cells. For infection assay, tissue filtrates were injected intraperitoneally to healthy, naive common carp. No virus was detected after three cell culture passages and the infection bioassays. One-step and nested-step PCR was used to detect KHV in gills of fish samples. One-step and semi-nested RT-PCR was used to detect SVCV and GCRV in the spleens, kidneys, and livers of fish samples. Samples from all three years from all five countries yielded negative results for all three viruses, indicating that KHV, SVCV, and GCRV were not present in these five countries during the period of the study although KHV outbreaks had been detected in Indonesia, Taiwan, Japan, Thailand, China, and Malaysia. -
Evaluation of hatchery-based enhancement of the mud crab, Scylla spp., fisheries in mangroves: comparison of species and release strategies
(CSIRO Publishing, 2009)Ranching, stock enhancement and restocking are management approaches involving the release of wild or hatchery-bred organisms to enhance, conserve or restore fisheries. The present study, conducted from April 2002 to November 2005, evaluated the effectiveness of releasing wild and hatchery-reared (HR) mud crabs in the mangroves of Ibajay, Aklan, Philippines where preliminary studies demonstrated declining fishery yields, abundance and size of crabs. Comparison of survival and growth of wild-released and HR Scylla olivacea and HR Scylla serrata demonstrated the effect of nursery conditioning, size-at-release and species differences. Overall yield and catch per unit effort (CPUE) increased by 46% after stock enhancement trials. Recapture rates of released crabs were highest in wild-released S. olivacea and in crabs measuring 65.0–69.9 mm carapace width (CW) and lowest in non-conditioned HR S. serrata. Growth rates were highest for conditioned HR S. olivacea and lowest for conditioned HR S. serrata (11.7 and 3.7 mm month-1 respectively). Fishing mortality was highest for S. olivacea, whereas natural mortality was greater for S. serrata. Conditioning hatchery-bred animals before release is also important in obtaining higher survival. S. olivacea was the more appropriate of the two species for release in mangrove habitats inundated with low-salinity water. However, there is a need for site-specific studies to evaluate the effectiveness of releases. -
Molecular epidemiology of koi herpesvirus
(Japanese Society of Fish Pathology, 2009)Three regions of koi herpesvirus (KHV) genomic DNA were compared for 34 samples from Japan, six from Indonesia, two from Taiwan, one from the Philippines, 13 from the Netherlands, one from the UK, one from the USA and one from Israel. The analyzed genomic regions included known PCR-detection targets (SphI-5, 9/5 and the thymidine kinase gene). The KHVs from Asian countries were very homogeneous, although two variants were noted based on a single nucleotide polymorphism. In contrast, seven variants were found in KHVs from outside of Asia, and although closely related to one another, they were clearly distinct from those from Asian. The results suggest that a clear genetic distinction exists between Asian and European (including each single isolate from the USA and Israel) types of KHV, and that unique types of KHV were independently introduced or emerged in the respective geographic locations. -
Non-linearity in ecosystem services: temporal and spatial variability in coastal protection
(Ecological Society of America, 2009)Natural processes tend to vary over time and space, as well as between species. The ecosystem services these natural processes provide are therefore also highly variable. It is often assumed that ecosystem services are provided linearly (unvaryingly, at a steady rate), but natural processes are characterized by thresholds and limiting functions. In this paper, we describe the variability observed in wave attenuation provided by marshes, mangroves, seagrasses, and coral reefs and therefore also in coastal protection. We calculate the economic consequences of assuming coastal protection to be linear. We suggest that, in order to refine ecosystem-based management practices, it is essential that natural variability and cumulative effects be considered in the valuation of ecosystem services. -
Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties
(Springer Verlag, 2009)Three varieties of Kappaphycus alvarezii (Kapilaran, KAP), Tambalang purple (PUR), Adik-adik (AA), and one variety of Kappaphycus striatum var. sacol (green sacol (GS) were used to determine the efficiency of Acadian marine plant extract powder (AMPEP) as a culture medium at different concentrations, for the regeneration of young plants of Kappaphycus varieties, using tissue culture techniques for the production of seed stock for nursery and outplanting purposes for the commercial cultivation of carrageenophytes. A shorter duration for shoot formation was observed when the explant was treated with AMPEP + Plant Growth Regulator (PGR = PAA + zeatin at 1 mg L−1) compared to AMPEP when used singly. However, four explants responded differently to the number of days required for shoot formation. The KAP variety took 46 days to form shoots at 3–4 mg L−1 AMPEP + PGR; while PUR required 21 days at 3–5 mg L−1 AMPEP and 3–4 mg L−1 AMPEP + PGR. AA required 17 days at 3–5 mg L−1 AMPEP and AMPEP + PGR; and GS 25 days at 1 mg L−1 AMPEP + PGR. It was observed that among the four explants used, PUR and AA initiated shoot formation with the use of AMPEP only at higher concentrations (3–5 mg L−1) after a shorter period. Only PUR responded positively to ESS/2 for shoot initiation. The use of AMPEP alone and/or in combination with PGR as a culture medium in the propagation of microplantlets using tissue culture technique is highly encouraging. -
Price dynamics and cointegration in the major markets of aquaculture species in the Philippines
(Southeast Asian Regional Center for Graduate Study and Research in Agriculture, 2009)This study explored the interdependencies of aquaculture markets in the Philippines by establishing price cointegration between wholesale and retail prices of three major species commonly farmed in the country, i.e., milkfish, tilapia and shrimp. The co-movements of wholesale prices between and among key markets for each species were also investigated. Moreover, exogeneity in prices was established using Granger-causation model to determine the existence of price leaders among these markets. Such information are crucial in farm management decisions of producers and traders through better understanding of the efficiency in price formation across production and consumption centers which in turn defines the movements in prices and products among markets. Appropriate policies for the development of markets for the three aquaculture species were also identified. Such policies are expected to contribute towards the attainment of efficient pricing and distribution of benefits among market players and stakeholders. These benefits are expected to manifest through the system of grading standards for fish traded in local markets and in the choice of cost-effective technologies in grow-out and post-production practices. -
Grow-out of juvenile seahorse Hippocampus kuda (Bleeker; Teleostei: Syngnathidae) in illuminated sea cages
(Blackwell Publishing, 2009)This paper examines the feasibility of rearing 10–15-day- and 0.7–1.5-month-old seahorse Hippocampus kuda in illuminated sea cages to continue existing hatchery protocols to mass produce H. kuda for trade and enhance depleted wild stocks in their natural habitats. Thawed Acetes (a planktonic crustacean abundant in inshore seas) was fed to juvenile seahorses in lighted and unlighted sea cages while one group in lighted cages was not fed Acetes. After 10–12 weeks of rearing, both mean body weight and stretch height increased in all treatment groups, with lighted cage-reared seahorses fed Acetes being heavier (2 g) and longer (8 cm) than the other two treatment groups. Although instantaneous growth rates declined during the rearing period, these were generally higher among Acetes-fed seahorses in lighted cages (0.02–0.07) compared with those in the unlighted cages with Acetes and lighted cages without Acetes feeding. Mean survivorship in all groups ranged from 9% to 74% after the trials, but mean survivorship of juveniles in lighted cages with Acetes feeding (9–74%) was consistently lower than the two treatment groups as a likely result of crustacean and piscine predators being attracted by light and the odour of leftover Acetes in the lighted cages. These results demonstrate that light-attracted zooplankton prey supplemented by Acetes feeding may provide essential nutrients for the growth of H. kuda juveniles in illuminated sea cages. With further improvement in the grow-out protocol, it may provide a possible alternative livelihood to seahorse fishers and sufficient seed to re-populate depleted wild stocks of H. kuda. -
Enrichment potential of HUFA-rich thraustochytrid Schizochytrium mangrovei for the rotifer Brachionus plicatilis
(Elsevier, 2009)An enrichment experiment was performed to evaluate the changes in lipid and essential fatty acid contents of the rotifer Brachionus plicatilis fed with freeze-dried cells of tropical thraustochytrid Schizochytrium mangrovei (Isolate IAo-1). Rotifers starved for 24 h were fed with S. mangrovei cells at 200, 300, 400, 500, 600 and 700 mg L−1. Enrichment was carried out at two periods (Short-term = 5 h; Long-term = 10 h) to determine the optimum time needed for the maximum enrichment of the rotifers. There was an overall significant increase in the total lipid, arachidonic acid (AA) and docosahexaenoic acid (DHA) contents of rotifers after feeding with freeze-dried S. mangrovei indicating the successful uptake of these nutrients in the rotifer's biochemical composition. On the other hand, docosapentaenoic acid (DPA) did not change significantly in enriched rotifers. Results of the present study indicate that both factors, feeding concentrations and enrichment periods, significantly affected the lipid, AA and DHA contents of rotifers. Uptakes of lipid, AA and DHA significantly increased with increasing feeding concentrations except for those fed the highest feeding concentration of 700 mg L−1 for 10 h. Moreover, lipid and AA contents of enriched rotifers were significantly higher during the short-term enrichment period while DHA contents were significantly higher during the long-term enrichment period. Therefore, it is concluded that the feeding concentration of 700 mg L−1 at an enrichment period of 5 h is optimum in the AA and DHA enrichment of rotifers. The strategic scheme of combining the proper amount of enrichment product and the duration of enrichment in boosting the DHA contents of rotifers will effectively ensure a reliable production of nutritionally superior rotifers at a minimal cost. This will ultimately contribute to the success of rearing marine fish larvae in the hatchery. -
Cage culture of the Pacific white shrimp Litopenaeus vannamei (Boone, 1931) at different stocking densities in a shallow eutrophic lake
(Blackwell Publishing, 2009)Postlarvae of Litopenaeus vannamei were acclimated and stocked in lake-based cages at the following stocking densities: 10, 20, 30 and 40 shrimp m−2. Another set of shrimp was stocked in concrete tanks as reference samples at 30 shrimp m−2. Significant differences were observed among stocking densities throughout the 95-day culture. The final weight at harvest decreased with increasing stocking density: mean weights of 23.3, 15.8, 13.0, 10.9 and 14.6 g for the 10, 20, 30, 40 shrimp m−2 and reference tanks were observed respectively. There were no significant differences in survival throughout the culture period, ranging between 69% and 77%. Daily growth rates (range: 0.11–0.24 g day−1) and specific growth rates (range: 3.54–4.34%) also differed significantly among stocking densities, both increasing with decreasing stocking density. The feed conversion ratio in the cages did not differ among the stocking densities, ranging from 1.53 to 1.65. The relationship between stocking density and mean individual weight at harvest followed the equation \(y=81.06x^{-0.54} (R^{2}=0.938)\) and that of stocking density and production (in g m−2) is \(y=58.01x^{−0.46} (R^{2}=0.834)\). -
Microsatellite and mitochondrial haplotype diversity reveals population differentiation in the tiger shrimp (Penaeus monodon) in the Indo-Pacific region
(International Society for Animal Genetics, 2008)The black tiger shrimp (Penaeus monodon) is an ecologically and economically important penaeid species and is widely distributed in the Indo-Pacific region. Here we investigated the genetic diversity of P. monodon (n = 355) from eight geographical regions by genotyping at 10 microsatellite loci. The average observed heterozygosity at various loci ranged from 0.638 to 0.743, indicating a high level of genetic variability in this region. Significant departures from Hardy–Weinberg equilibrium caused by heterozygote deficiency were recorded for most loci and populations. Pairwise FST and RST values revealed genetic differentiation among the populations. Evidence from the assignment test showed that the populations in the West Indian Ocean were unique, whereas other populations examined were partially admixed. In addition, the non-metric multidimensional scaling analysis indicated the presence of three geographic groups in the Indo-Pacific region, i.e. the African populations, a population from western Thailand and the remaining populations as a whole. We also sequenced and analysed the mitochondrial control region (mtCR) in these shrimp stocks to determine whether the nuclear and mitochondrial genomes show a similar pattern of genetic differentiation. A total of 262 haplotypes were identified, and nucleotide divergence among haplotypes ranged from 0.2% to 16.3%. Haplotype diversity was high in all populations, with a range from 0.969 to 1. Phylogenetic analysis using the mtCR data revealed that the West Indian Ocean populations were genetically differentiated from the West Pacific populations, consistent with the microsatellite data. These results should have implications for aquaculture management and conservation of aquatic diversity. -
Distribution and symptoms of epiphyte infection in major carrageenophyte-producing farms
(Springer Verlag, 2008)High density commercial farming of carrageenophyte Kappaphycus alvarezii is often plagued with “ice-ice” disease and epiphyte infection, which eventually leads to reduced production and in some cases collapse of crop. Epiphyte outbreak has been occurring regularly in major carrageenophyte farms in the Philippines, Indonesia, Malaysia and Tanzania. Infected materials from these countries were studied to establish baseline information on the epiphyte’s identity, density, symptoms and secondary infection on the host seaweed. The causative organism was identified as Neosiphonia apiculata (Hollenberg) Masuda et Kogame, based on its morphological features. Epiphyte density on host seaweed materials decreased in the following order: the Philippines (88.5 epi cm-2), Tanzania (69.0 epi cm-2), Indonesia (56.5 epi cm-2) and Malaysia (42.0 epi cm-2). Initial symptoms were the presence of tiny black spots, indicating the embedded tetrasporeling in seaweed cortex layer. Vegetative form emerged after 2 weeks measuring less than 0.5 mm in length with a density of less than 25.0 epi cm-2. Upon maturation, infected seaweed takes on a “hairy” appearance with “goose-bumps” like cortical swellings. The epiphyte appears as a solitary plant with multiple secondary rhizoids or as multiple epiphytes appearing from a single cortical opening. At the end of infection, the epiphytes left dark pits on the cortical swelling, and the carrageenophytes are infected by opportunistic bacteria. Bacterial enumeration of healthy and infected seaweed materials showed an increase of more than 300% in total bacterial count on infected materials dominated by Alteromonas sp., Flavobacterium sp. and Vibrio sp. -
Indicators of diminished organic matter degradation potential of polychaete burrows in Philippine mariculture areas
(University of the Philippines Los Baños, 2008)Sediments from underneath fish cages and in mesocosm tanks were examined to establish indicators of diminished organic matter degradation potential of polychaete burrows from increased mariculture activities in Bolinao-Anda, Pangasinan, Philippines. Results showed that simple sediment characteristics may be used as sediment quality indicators to describe the contribution of burrows to biodegradable processes in sediments with extremely high deposition of organic particles. The indicators of diminished organic matter degradation potential of polychaete burrows are low redox potential (-113 to -150 mV for F. Spionidae and F. Eunicidae) at the 1 cm surface layer, absence or decreased size of burrow openings, presence of H sub(2)S, disappearance of apparent Redox Potential Discontinuity Layer (aRPD), formation of black sediment, and presence of Beggiatoa (sulfide oxidizing bacteria) and gas bubbles. -
Low isozyme variation in native and transplanted populations of the endemic Philippine silver perch, Leiopotherapon plumbeus (Kner, 1864) from three lakes in the Philippines
(College of Agriculture, University of the Philippines Los Baños, 2008)The silver perch, Leiopotherapon plumbeus (Kner, 1864), is an endemic and economically important fish in the Philippines. A native species of Laguna de Bay, the silver perch was transplanted from this lake to Sampaloc Lake in the late 1950s and Taal Lake in the early 1970s. Morphological variation has been observed among the native and transplanted populations of the silver perch. In this study, genetic variation within and between the native and transplanted populations was examined using isozyme electrophoresis. Four sites were sampled: two in Laguna de Bay (Binangonan and Tanay) and one each in Sampaloc Lake and Taal Lake. Twenty-two (22) loci were scored but only one locus (PGM*) was polymorphic by the 95% criterion and only two loci (ADH* and PGM*) were polymorphic by the 99% criterion. The average heterozygosities, genetic distances and FST values of specimens from the four sites were very low, suggesting that the native and transplanted populations had very low genetic variation and that they were genetically homogeneous despite the presence of morphological varia- tion. The low genetic variation could possibly be due to a population bottleneck in the native population in Laguna de Bay in the past and to the low genetic variation of the founders in the transplanted populations. -
Enhancing nonspecific immune response of grouper, Epinephelus coioides using levamizole as immunostimulant
(University of the Philippines in the Visayas, 2008)The use of levamisole as an alternative approach to disease prevention and control has been resorted to in aquaculture. Its immunomodulatory effect in several fish species is well documented but its effect on grouper, Epinephelus coioides requires further investigation. To determine its immunomodulatory effect on grouper, juveniles were injected and fed with levamisole. Intraperitoneal injection (IP) at 0 (control), 0.5, 5, 10, 20 and 50 mg levamisole/kg body weight was administered. Grouper were also fed diets containing 0 (control), 125, 250 and 500 mg levamisole/kg supplemental dry diet for 10 days. Hematocrit levels, total and differential leucocyte counts and bactericidal activity were examined 7 days post IP. Three, 5 and 7 weeks after last administration of supplemented feeds, growth and bactericidal activity werie checked. A significant (P<0.05) increase in the total leucocyte count was noted in the injection-treated fish. Granulocyte (19.39%) and monocyte (27.38%) percentages increased while lymphocyte(53.40%) population decreased in the differential leucocyte count. However, hematocrit levels were not affected by levamisole. Bactericidal activity against Vibrio anguillarum increased in treated (injected and supplemented) fish. A significant (P<0.05) increase in weight of juvenileswas observed 10 days after last feeding with supplemented diet. Groupers injected with 0.5 mg levamisole/kg body weight and those supplemented with 125 mg levamisole/kg dry diet exhibited the most significant (P<0.05) changes in parameters tested indicating immunostimulation. Growth (weight gain) and bactericidal activity peaked 5 weeks post administration of levamisole in the feeding experiment. Results of the present study strongly suggest that levamisole can enhance nonspecific defenses in grouper and opens the possibility of using immunostimulants in grouper culture.





















