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Detection and identification of viral pathogens of grouper (Epinephelus malabaricus) cultured in Thailand
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
A virological survey was conducted in brown-spotted grouper (Epinephelus malabaricus) cultured in cages, earthen ponds and pens from April 2001 to January 2002 on the east and south coasts of Thailand. The adult grouper ...
Prevalence of economically-important viral diseases in wild shrimp (Penaeus monodon) in the Philippines
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Prevalences of white spot syndrome virus (WSSV) and monodon baculovirus (MBV) were determined using polymerase chain reaction (PCR) as the detection method in wild black tiger shrimp (Penaeus monodon) collected from seven ...
Standardization of diagnostic methods for monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV): Establishment of monoclonal antibodies (MAbs) against MBV and HPV
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) remain as two of the serious pathogens that infect black tiger shrimp (Penaeus monodon) in the Philippines. It is, therefore, necessary to prevent the spread ...
Production of polyclonal antibodies (PAbs) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) are two of the viral pathogens, which infect penaeid shrimps in the Philippines. Polyclonal antibodies (PAbs) to MBV and HPV were raised by immunization of ...
Detection and transmission of the koi herpesvirus (KHV)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
The isolations of KHV were conducted on samples collected from Indonesia, Taiwan and Vietnam. No virus was isolated from koi and common carp from the first two countries although PCR tests of samples from Taiwan were ...
Detection and identification of viral pathogens in marine finfish cultured in the Philippines
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
This is a summary of investigations conducted on 91 disease/mortality cases observed among marine fish: groupers (Epinephelus coioides, E. malabaricus), sea bass (Lates calcarifer), rabbitfish (Siganus spp.), red snapper ...
Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Several runs were conducted using the three published PCR protocols and the three DNA extraction methods. All three primer pairs designed by individual authors based on the sequence of a cloned fragment of the white spot ...
Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia: General information
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Research is the main component of the Regional Fish Disease Project supported by the Government of Japan (GOJ) Trust Fund. When the project started in 2000, research was undertaken by scientists of the SEAFDEC Aquaculture ...