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Development of shrimp (Penaeus monodon) cell culture in vitro
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Primary shrimp cell cultures were successfully developed from lymphoid organ of black tiger shrimp (Penaeus monodon) in double-strength Leibovitz’s L-15 medium supplemented with 15% fetal bovine serum, 1% glucose, 15% ...
Standardization of diagnostic methods for monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV): Establishment of monoclonal antibodies (MAbs) against MBV and HPV
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) remain as two of the serious pathogens that infect black tiger shrimp (Penaeus monodon) in the Philippines. It is, therefore, necessary to prevent the spread ...
Production of polyclonal antibodies (PAbs) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) are two of the viral pathogens, which infect penaeid shrimps in the Philippines. Polyclonal antibodies (PAbs) to MBV and HPV were raised by immunization of ...
Research and other activities of the SEAFDEC Aquaculture Department for koi herpesvirus (KHV)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Development of monoclonal antibody (MAb) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Antibody-based detection methods are relatively simple and rapid, aside from being specific and sensitive. Thus, there is a necessity to develop such techniques for early and rapid diagnosis of viral pathogens such as MBV ...
Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Several runs were conducted using the three published PCR protocols and the three DNA extraction methods. All three primer pairs designed by individual authors based on the sequence of a cloned fragment of the white spot ...
Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia: General information
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Research is the main component of the Regional Fish Disease Project supported by the Government of Japan (GOJ) Trust Fund. When the project started in 2000, research was undertaken by scientists of the SEAFDEC Aquaculture ...
Studies on the experimental transmission model of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Series of experiments on the transmission of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon were undertaken. Feeding of MBV-infected carcasses to experimental shrimps resulted to 100% ...