Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff: Recent submissions
1501-1520 / 1693
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Urease activity in soybean meal: effect on its nutritional quality and on growth and survival of Penaeus monodon juveniles.
(International Foundation for Science, 1998)Based on the results of a survey conducted on the urease activity (UA) in commercial shrimp feed containing soybean meal (SBM) (0.00-29. 70 ppm UA), a study was carried out to determine the effects of different heat treatments on the UA, and on the nutritional quality of SBM. The effect of these heat treated SBMs when incorporated into shrimp diet on the growth and survival of Penaeus monodon juveniles was likewise tested. Various levels of UA in SBM were obtained with different heat treatments. Six practical diets were formulated and contained 0.00 (SBM heated at !20°C for 20 min); 0.50 (SBM heated at 60°C for 160 min); 4.0 (SBM heated at 60°C for 80 min); 8.0 (SBM heated at 60°C for 40 min); 11.0 (SBM heated at 60°C for 20 min) and 22.0 ppm UA (without heating). These diets were fed to P. monodon (average weight = 4.24±0.10 g) juveniles for a period of 60 days. Results showed that protein quality in terms of amino acid content of SBM was not significantly affected by the different heat treatments. Weight gains of shrimps fed diets with 8.0, 11.0, 25.0 ppm UA were significantly lower than those fed other diets. Survival of shrimps was lowest with diets containing unheated SBM, but this was not significalltly different from those heated at 60°C. Heat treatment of SBM at 120°C is adequate to be an effective ingredient in shrimp diets. -
Zooplankton distribution during winter using Two-Way Indicator Species Analysis (TWINSPAN) and Canonical Correspondence Analysis (CANOCO).
(1998)Spatial distribution and community structure of zooplankton species and its relationships with the environmental parameters (temperature, salinity, and turbidity and phytoplankton distribution) in some sandbanks of the Belgian coastal zone was carried out by the use of Two-Way Indicator Species Analysis (TWINSPAN) and Canonical Correspondence Analysis (CANOCO). There were 49 zooplankters identified all over the three sandbanks. Crustaceans was the most dominant sub-phyla represented mostly by copepods. Other members of plankton community were also present like cladocera, appendicularia, pteropoda and the larger omnivores and carnivores such as hyperiid, amphipod, chaetognath, ctenophore and fish larvae. TWINSPAN did not show a distinct division of the sandbank stations because of the presence of three dominant copepod species (Temora longicornis, Pseudocalanus elongatus and Centropages hamatus) in the three sandbanks. Canonical correspondence analysis (CANOCO) showed that species distribution of zooplankton was significantly affected by the environmental variables. This was due to lower salinity values observed around Buitenratel sandbank than Gootebank and Westhinder sandbanks. Two-Way Indicator Species Analysis analyzed spatial distribution and community structure of zooplankton species over the three sandbanks of the Belgian coastal zone based on species abundance. Canonical Correspondence Analysis (CANOCO) on the other hand, gave an idea on how far these subgroups were related to environmental variables. -
Historical and current trends in milkfish farming in the Philippines
(Academic Press, 1998)This chapter focuses on the historical and current practices of milkfish farming in the Philippines. The Philippines ranks among the top 12 largest fish producers in the world and the milkfish, Chanos chanos, is the official national fish. The milkfish production in the Philippines has fluctuated sharply, but on average, has relatively stagnated over the past decade, partly due to the shrimp boom and low price of milkfish. The milkfish industry has been responsible for the significant loss of valuable mangrove swamps and forests. The loss of mangrove means loss of habitats and biodiversity including nursery grounds for feeding and refuge of commercial fishes, shrimps, crabs and mollusks. Milkfish ponds in the Philippines are either privately owned or leased from the government. Brackish water fish ponds are valuable real estate and good management adds to their value. For milkfish farming, stocking rate should be based on the pond environment and carrying capacity, and the fish size at stocking and the market size desired. -
Protein and dry matter digestibility of feedstuffs in complete diets for Penaeus monodon
(Asian Fisheries Society, 1997)The digestibilities of animal and plant protein sources were determined for P. monodon (30 ~ 40 g wet weight). Protein sources were incorporated in complete diets containing the indicator, chromic oxide at 1.8%. Casein, (APDC=97.9%) was highly digested. The APDC values of shrimp meal (Acetes sp.) and squid meal were 95.4% and 96.0%, respectively. These values did not differ from those for plant protein sources such as soybean meal (93.4%), yeast (93.0%) and wheat germ meal (91.9%). Fish meal (60.75%), meat and bone meal (73.8%) and copra meal (75.2%) had significantly lower APDC values while shrimp head meal and testis meal were digested at 89%~90%. Assimilation (U1) of dietary organic matter by the ash-free method ranged from 58.3%~90.19%. The TPDC of feedstuffs were generally higher by about 5% than the APDC. A highly positive correlation was observed between APDC and ADMDC (r=90). Protein content of all diets which ranged from 12.9%~60.18% showed no correlation with APDC, TPDC, ADMDC and U1. This study showed that the shrimp has the capability to similarly digest plant and animal protein sources. -
Comparative strategies in community-based mangrove rehabilitation programs in the Philippines
(United Nations Educational Scientific and Cultural Organisation; Japanese Man and the Biosphere National Committee; Mangrove Ecosystem Research Centre, 1997)Philippine Mangroves have decreased from around 500,000 ha at the turn of the century to 132,000 ha in 1990. Given the varied and important socioeconomic and ecological functions of mangroves including harvest of forestry and fishery products, coastal protection, erosion control and pollution abatement, there is a need to rehabilitate degraded areas. Half of the mangrove areas lost in the las three decades can be traced to the construction of 141'000 ha of brackish water ponds monocropped to mik fish or shrimp. Integrated aquasilviculture is an alternative, although experimental, technology that combines both the fisheries and forestry functions of the mangrove ecosystem.This paper describes four models of mangrove rehabilitation and conservation with varying degrees of community participation in the philippines-monoculture mangrove planting in Kalibo, Aklan; mixed species planting in Silay City, Negros Occidental; aquasilviculture in Puerto Galera, Mindoro Oriental (family) and in Hinobaan, Negros Occidental (association)- and compares their strengths, weaknesses, opportunities and threats. -
Optimum stocking density and tank size for larval rearing of the grouper, Epinephelus coioides
(Asian Fisheries Society, 1997)The effect of stocking of stocking density and tank size on early growth and survival of grouper, Epinephelus coioides (=suillus) larvae were determined. In one experiment, larvae were stocked in 500L tanks at densities of 5, 10, 20 and 30 per liter and fed rotifers at 15 ind/mL. After 21 d, larvae attained best growth (9.6 mm) and highest survival (32.2%) at 20/L. In another experiment, larvae were stocked at 30/L in 40 L, 200 L and 500 L circular fiberglass tanks. Survival was highest in 500 L (22.3%) and lowest in 40 L tanks (0.5%), but larvae in 200 L tanks were larger (8.2 mm total length, 1.5 mg dry weight) on day 21. Thus in small tanks, 20 larvae/L is the optimum stocking density. In larger tanks of 500 L, 30 larvae/L is feasible. -
A strategy for sustainable mariculture
(Malacca Straits Research and Development Centre (MASDEC), Universiti Putra Malaysia, 2002)From merely growing shellfish and macroalgae along the freinges of the sea, man is naw growing marine fish in cages thus expanding the range of mariculture. While the farming of shellfish may have its own set of problems it is the growing of fish that particularly poses more serious problem. This is especially true since high value fish which invariably are carnivores are the preferred species. Depending on the hydrography of the site, the species culture, culture method, stocking density, feedtype, and husbandry practices marine fish farming has the potential of greatly increasing the organic load of the immediate waters. the use of chemicals such as therapeutants, vitamins, pigments, and anti-foulants futher adds to the pollution. If the rate and manner of development is left to market forces its sustainability may be jeopardized. Areas that are semi-enclosed are often preferred and can rapidly become subject to overcrowding to the extent that its carrying capacity is exceeded. In addition conflicts between growersand other users can arise thus adding a social dimension to the problem. Sustainability of mariculture can be better assured if the development is not haphazard. The concept of zoning and planned development which is now the standard appeoach on land can wery well be applied in the sea. The open waters can be zoned as to type of aquaculture allowable. Beyond zoning, growers can be induced to set up their cages in semi-exposed or even exposed areas where th hydrography is more ideal for growing fish by providing an infrastructure for mariculture. Such infrastructure may consist of nothing more than mooring facilities for deep-watercages and, where necessary, floating breakwaters. With proper spacing of the mooring points, even the density of the cages in an area can be regulated. This will effectively lower start-up cost since growerswill be spared the expense of installing mooring. This will make it possible to broaden the participation to those with limited access to capita such as small-sscale fishers. Users can be charged mooring fees much as ships are charged berthing fees. Service in the form of security may also be provided. this can be complimented by onshor facilities such as a landing area, feed depot, ice plant, cold storage and processing plant. Such an area can be operated like a marine version of an aindusrial park and can aptly be referred to as a mariculture park. This model of mariculture development is being tried in the philippines. -
Viability of milkfish eggs and larvae after simulated and actual transport
(Physiology Section, American Fisheries Society, 1996)The viability of milkfish eggs and larvae after simulated and actual transport was investigated. Naturally-spawned milkfish eggs were collected and subjected to simulated or actual transport at early cleavage stage (stage 1), blastula (stage 2), gastrula (stage 3), "eyed" (stage 4), or newly-hatched larvae (stage 5). Replicate samples in aerated plastic jars served as controls. Mean hatching and survival rates and the percentage of newly-hatched larvae were significantly affected by the modes of transport and by the stage of embryonic development at transport. Eggs transported at the 'eyed' stage had higher viability compared to those transported at cleavage, blastula, or gastrula stages. There was no significant difference in the mean survival rate of the larvae after 26 days of rearing. However, the percentage of 45 day old larvae with apparent morphological abnormalities was lower in groups transported at stages 4 and 5. These observations indicate that milkfish eggs should be handled and transported during the late embryonic stages to minimize mortalities and the incidence of abnormalities in larvae. -
Egg quality of grouper Epinephelus coioides fed different fatty acid sources
(American Fisheries Society, Physiology Section, 1996)Quality of eggs spawned by Epinephelus coioides fed fish by catch (control). Cod liver oil-enriched fish by catch (TFC), and commercial HUFA A-enriched fish by catch (TFS) was monitored. Monthly egg production, spawning frequency, fertilization rate, egg viability, and hatching rates of the control were significantly higher compared to TFS Egg production. Spawning frequency and hatching rate of TC and TFS were not significantly different. Results suggest that varying the species of fish by catch could provide the requirements of E. Coioides broodstock so as to provide quality eggs. -
The status of seed production of grouper and other coral reef fishes in the Philippines
(Institute for Development Studies, 1999)Initial work on seed production of grouper in the Philippines was first reported in 1986. Most of the work has been undertaken by the SEAFDEC Aquaculture Department (SEAFDEC/AQD), which concentrated on Epinephelus coioides. The achievements and results of SEAFDEC/AQD on breeding and larval rearing of grouper which including studies on sex inversion will be discussed. Directions for future research directions are also presented. -
Shrimp health research in the Asia-Pacific: present status and future directives
(Food and Agriculture Organization of the United Nations, 1996)Shrimp harvests from intensive aquaculture have recently declined in areas which have been productive for many years. Because the most convenient, although not necessarily factual, explanation for these crop failures has been the occurrence of infectious diseases, there is a need to consider shrimp health from a holistic point of view. The classical method used for the study of shrimp disease dealt mainly with identification of the causative organism and the search for methods of prevention and control through chemotherapy. The adverse affects resulting from the use of chemicals in aquaculture have led to a clamor for alternative approaches to disease management. For effective shrimp health maintenance and surveillance, the following components need consideration: development of rapid and sensitive methods for pathogen detection; establishment of shrimp tissue cultures for virology; immunological studies, toxicological studies and drug efficacy evaluation. The epidemiological approach to disease management should augment the classic approach to shrimp pathology, and this calls for multidisciplinary cooperation. -
Series: NACA Environment and Aquaculture Development Series No. 3
Gracilaria studies at SEAFDEC/AQD
(Network of Aquaculture Centres in Asia-Pacific, 1996)Research in seaweed from 1988–1994 focused on the agar producing Gracilaria. Studies in the following areas were also conducted: inventory of the seaweed resources; production ecology; farming systems; and agar characterisation. Six species of Gracilaria and one Gracilariopsis abound in the Western Visayas. There were monthly variations in biomass and agar quality of G. blodgettii, G. manilaensis and G. heteroclada collected at different places. The bulk of the studies were carried out on Gracilariopsis heteroclada because of its wide distribution, fast growth characteristics and good quality agar. Its reproductive state was seasonal with tetrasporophyte abundant in May and carposporophyte in January. Seventy-five percent harvesting of the available biomass was sufficient for the next cropping season. Harvesting the seaweed using “araña” was not appropriate in places where the biomass was exposed to air during the lowest tide. The addition of nutrients to the stock increased the growth rate of the seaweed and gel strength of the agar. G. heteroclada grown at lower stocking density in hapa nets, both in floating cages and in ponds, gave higher growth rates and production than at higher stocking densities. This species, when polycultured with P. monodon at lower stocking density combinations, gave the highest growth rate and income. Likewise, this species when cultured vertically in ropes inside a floating cage showed encouraging results. G. heteroclada, when grown at 24–25 ppt under tank conditions, produced the highest gel strength. -
Management of fish health in broodstock and larvae of milkfish, sea bass and grouper
(The Oceanic Institute, 1996)Historically, reports on the occurrence of disease problems in milkfish, seabass and grouper were mainly on the isolation and identification of etiological agents. Studies on the tolereance of fish to chemotherapeutants were also conducted. Various species of vibrios and gill-infesting parasites have been associated with diseases in all life stages of these fish. Presently, mortalities due to diseases of unknown etiology, environmental failure and nutritional or husbandry shortfalls affect successful fry production in the hatchery. Morphological deformities in hatchery-produced milkfish fry is a major problem to be solved. In seabass fry production, swim-bladder stress syndrome occasionally occurs and results in mass mortalities. In larval grouper culture, the problem of low survival is being addressed through improved husbandry and nutrition. For cage-held broodstock that naturally spawn in captivity, such as milkfish and grouper, the main problem is fouling of net enclosures in the cages. However, for fish that need hormonal manipulation to spawn, such as seabass and snapper, stressful handling procedures during sampling and hormone injection may lead to injuries and scale loss. The damaged areas become focal points for secondary bacterial infection. Future fish health management strategies in these fish should incorporate a holistic approach to include environmental monitoring, nutritional manipulation, immunostimulants and vaccine use, and biological control in addition to pathogen exclusion in the rearing system and the fish. -
Maturation and induced spawning of the mangrove red snapper (Lutjanus argentimaculatus) reared in a floating net cage in the Philippines
(International Center for Living Aquatic Resources Management (ICLARM), 1996)Wild-caught mangrove red snapper (Lutjanus argentimaculatus) were reared in a floating net cage at SEAFDEC/AQD's Igang Marine Substation at Guimaras Island, Central Philippines. In 1993, monthly samplings and induced spawning trials were conducted as part of a project on seed production. Gonadal development began in February for males and April for females, reached a peak in September and declined in December. Males and females had ripe gonads for up to 5-6 consecutive months. Spawning occurred in the evening (2000 to 2300 hours), 32-36 hours after a single intramuscular injection of 1,500 IU human chorionic gonadotropin kg-1 body weight (BW). About 0.53-2.14 million eggs were collected per female (2.5-4.4 kg BW) with fertilization rates of 21-97% and hatching rates of 42-80% resulting in 30-85% normal larvae (straight and without deformities). The results strongly suggest the feasibility of breeding mangrove red snapper in floating net cages. -
Use of metaldehyde as molluscicide in milkfish ponds
(British Crop Protection Council, 1996)METAR metaldehyde formulations were tested under laboratory and faild conditions againts brackishwater pond snails (certhium so.). Under laboratory conditions the LC50 and LC99 3 days after treatment ranged from 2 - 3.5 and 4.8 - 5.4 kg/ha, respectively. However. these levels proved inffection when applied directly under actual pond conditions. In ponds with snail populatin of about 300/m2, a higher application rate of 30 kg/ha is recommended. Application of META metaldehyde concentrations of 0 - 175 kg/ha did not affect milkfish juveniles (1 - 3g body weight) 7 days after treatment. Results suggest that META metaldehyde formulations were effective for pond snail control without detrimental effect on juvenile milkfish. -
Order Gonorynchiformes: Chanidae: Milkfish
(Food and Agriculture Organization of the United Nations, 1999) -
Technology transfer and information dissemination at SEAFDEC/AQD.
(ASEAN-EEC Aquaculture Development and Coordination Programme, 1995) -
Assessment of the seaweeds industry
(Department of Science and Technology and United Nations Development Programme, 1995) -
Series: Special Publication; No. 24
Improved hatchery rearing techniques for the Asian catfish, Clarias macrocephalus (Gunther)
(European Aquaculture Society, 1995)The Asian catfish, Clarias macrocephalus, is a popular freshwater foodfish in the Philippines. However, grow-out farming of this species is hampered by the lack of juveniles for stocking. A series of experiments on zooplankton feed density, weaning and stocking density were conducted in order to improve production of C. macrocephalus fry in the hatchery. -
Siderophore detection among bacteria associated with the epizootic ulcerative syndrome (EUS)
(Fish Health Section, Asian Fisheries Society, 1995)Sixteen isolates of Aeromonas hydrophila andone isolate each of Aeromonas sp. n., Aquaspirillum sp., Pseudomonas sp., and Streptococcus sp., isolated from normal, apparently normal and epizootic ulcerative syndrome (EUS)-affected fish were screened for siderophore production at 20, 25, 30, and 37oC. results showed that siderophore production of A. hydrophila and Aeromonas sp. n. isolates decreased with increasing temperature. Among A. hydrophila isolates, 81.2% produced siderophore at 20oC, 50% at 25 and 30oC, and only 37.5 at 37oC. In contrast, Aquaspirillum sp., Pseudomonas ep., and Streptococcus sp., showed higher production of siderophore at 30 and 37oC.



















