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Standardization of PCR techniques as the detection method for koi herpesvirus (KHV) infection in koi and common carp
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Several runs have been made using the two published PCR protocols in the detection of koi herpesvirus (KHV). The primer pairs designed by individual authors based from the sequence of a cloned fragment of KHV genome ...
Establishment of preventive measures against viral nervous necrosis (VNN) in finfish hatcheries
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
The establishment of preventive measures against viral nervous necrosis (VNN) at the finfish hatcheries of SEAFDEC Aquaculture Department in Iloilo, Philippines, was instituted primarily to minimize the impact of the viral ...
Chlorine disinfection against hepatopancreatic parvovirus (HPV) infection in shrimp (Penaeus monodon) larvae
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Experiments were undertaken to determine the efficacy of chlorine as a disinfectant in inhibiting HPV infection among P. monodon postlarvae (PLs). Based on histopathological examination, 50 ppm and 200 ppm active chlorine ...
Use of bacteria as biological control agent against microbial diseases in shrimp (Penaeus monodon) and crab (Scylla serrata) hatcheries: Part III. Application of probiotics in culture water and surfaces
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Strain C1 is a bacterial isolate, which can reduce luminous bacteria in seawater, and thus a potential probiotic in crab larval rearing system. A delivery method through colonized substrates like plastic beads, window pane ...
Use of bacteria as biological control agent against microbial diseases in shrimp (Penaeus monodon) and crab (Scylla serrata) hatcheries: Part I. Isolation and selection of bacteria for biological control in crustacean hatcheries
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
A study aimed at obtaining a biological control agent against bacterial diseases of hatchery-reared shrimps and crabs was conducted. Bacteria were isolated from crustacean rearing environments where luminescent vibriosis ...
Prevalence of economically-important viral diseases in wild shrimp (Penaeus monodon) in the Philippines
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Prevalences of white spot syndrome virus (WSSV) and monodon baculovirus (MBV) were determined using polymerase chain reaction (PCR) as the detection method in wild black tiger shrimp (Penaeus monodon) collected from seven ...
Standardization of diagnostic methods for monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV): Establishment of monoclonal antibodies (MAbs) against MBV and HPV
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) remain as two of the serious pathogens that infect black tiger shrimp (Penaeus monodon) in the Philippines. It is, therefore, necessary to prevent the spread ...
Production of polyclonal antibodies (PAbs) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) are two of the viral pathogens, which infect penaeid shrimps in the Philippines. Polyclonal antibodies (PAbs) to MBV and HPV were raised by immunization of ...
Use of bacteria as biological control agent against microbial diseases in shrimp (Penaeus monodon) and crab (Scylla serrata) hatcheries: Part II. Application of probiotics in natural food and artificial feeds
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
The suitability of probiotic bacterial incorporation into artificial diets was tested with inferior results due to the interference of heat-resistant starins that are associated with several feed ingredients. However, ...
Development of immunological indices for monitoring health status in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Some hemolymph parameters were determined as non-invasive early-warning indicators of health status in black tiger shrimp (Penaeus monodon). These included total hemocyte count (THC), respiratory burst activity, phenoloxidase ...