Southeast Asian Fisheries Development Center, Aquaculture Department Institutional Repository (SAIR) is the official digital repository of scholarly and research information of the department. This is to enable the effective dissemination of AQD researchers' in-house and external publications for free and online. The repository uses DSpace, an open source software, developed at Massachusetts Institute of Technology (MIT) Libraries. It is an Open Archives Initiative (OAI)-compliant.

Initially, the repository shall contain preprints, full-texts or abstracts of journal articles, books and conference proceedings written by SEAFDEC/AQD scientists and researchers. The aim is to promote these publications especially those published in international peer-reviewed journals and generate higher citation through increased visibility.

It will also provide free and open access to all in-house publications of SEAFDEC/AQD. Full-text digitized copies of fishfarmer-friendly materials like books, handbooks, policy guidebooks, conference proceedings, extension manuals, institutional reports, annual reports (AQD Highlights), and newsletters (SEAFDEC Asian Aquaculture, Aqua Farm News, AquaDept News and AQD Matters) can be retrieved and downloaded.

In the future, SAIR will expand its collection to include images, presentations, audios, and videos among others.

The objectives of the repository are to: (1) to provide reliable means for SEAFDEC/AQD researchers to store, preserve and share their research outputs and (2) to provide easy access and increase the visibility of SEAFDEC/AQD scientific publications

SAIR also aims to encourage SEAFDEC/AQD researchers for self-archiving and submitting pre-prints from which metadata will be screened and approved by the library staff.

  • Article

    Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos 

    BS Santos, MRR Romana-Eguia, ZU Basiao & M Ikeda - Conservation Genetics Resources, 2015 - Springer Verlag
    The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.

Select a community to browse its collections.

  • Article

    Gonadal response of juvenile protogynous grouper (Epinephelus fuscoguttatus) to long term recombinant follicle-stimulating hormone administration 

    P Palma, J Nocillado, J Superio, EGdJ Ayson, F Ayson, I Bar & A Elizur - Biology of Reproduction, 2018 - Oxford University Press
    The role of follicle stimulating hormone (FSH) in the gonadal development of protogynous hermaphroditic grouper (E. fuscoguttatus) was investigated. Recombinant giant grouper (E. lanceolatus) FSH (rggFSH) was produced in yeast. Its receptor binding capacity and steroidogenic potency were confirmed in vitro. Weekly injections of rggFSH to juvenile tiger grouper for 8 weeks (100 μg/kg body weight, BW) resulted in significantly larger and more advanced oocytes (cortical alveolar stage vs. primary growth stage in control). Sustained treatment with rggFSH (20 to 38 weeks at 200 μg/kg BW) resulted in significant reduction in gonad size, degeneration of oocytes and proliferation of spermatogonial cells, indicative of female to male sex change. Gene expression analysis showed that, while initiating female to male sex change, the rggFSH significantly suppressed the steroidogenic genes cyp11b, cyp19a1a and foxl2 which restrained the endogenous production of sex steroid hormones thus prevented the differentiation of spermatogonial cells. Expression profile of sex markers dmrt1, amh, figla and bmp15 suggests that the observed sex change was restricted at the initiation stage. Based on these results, we propose that the process of female to male sex change in the protogynous grouper is initiated by FSH, rather than sex steroids and likely involves steroid-independent pathway. The cortical alveolar stage in oocyte development is the critical point after which FSH-induced sex change is possible in grouper.
  • Article

    Immunization regimen in Asian sea bass (Lates calcarifer) broodfish: A practical strategy to control vertical transmission of nervous necrosis virus during seed production 

    R Pakingking Jr., EG de Jesus-Ayson, O Reyes & NB Bautista - Vaccine, 2018 - Elsevier
    Outbreaks of viral nervous necrosis (VNN) in Asian sea bass (Lates calcarifer) at the larval stages via vertical transmission of nervous necrosis virus (NNV) from asymptomatic broodfish remain as a major deterrent during seed production. A five-year study was conducted to produce NNV-specific-free sea bass broodfish reared in land-based tanks through an annual immunization regimen with the formalin-inactivated NNV. We primarily immunized (intraperitoneal injection) sea bass juveniles (5 g) and monitored the neutralizing antibody (Nab) titers in the sera of these fish at scheduled intervals post-immunization. Nab titers in the sera of immunized fish peaked at Month 2 (titer: 1:4480 ± 1185) but thereafter gradually declined and significantly dropped (1:260 ± 83) at Month 12 post-primary immunization. Booster immunization of these fish at Month 12 post-immunization led to abrupt increases in Nab titers in booster immunized (B-Im) fish at Month 1 (1:12800 ± 6704) but thereafter declined and dropped at Month 12 (1:480 ± 165) post-booster immunization. The annual booster injections with the inactivated vaccine or L-15 (Unimmunized [U-Im]) were consecutively conducted for 4 years until the fish became sexually mature. Mature fish from both groups were successively induced to spawn twice (1-month interval) via intramuscular injection with luteinizing hormone-releasing hormone analogue (LHRH-a; 100 µg/kg BW). NNV was not detected by RT-PCR in oocytes and milts, and spawned eggs of B-Im fish. In contrast, oocytes and milts, and spawned eggs of U-Im fish were NNV positive. Spawned eggs of B-Im broodfish exhibited Nab titers ranging from 1:192 ± 34 to 1:240 while such was not detected (<1:40) in eggs of U-Im fish. Taken together, current data clearly demonstrate that annual immunization regimen with inactivated NNV vaccine is a pragmatic approach for sustaining immunocompetent sea bass broodfish reared in land-based tanks and circumvent the risk of vertical transmission of NNV from asymptomatic broodfish to their offspring under stress of repetitive spawning.
  • Article

    Limit of colour vision in dim light in larvae of the giant freshwater prawn Macrobrachium rosenbergii 

    G Kawamura, TU Bagarinao, ASK Yong, AB Faisal & LS Lim - Fisheries Science, 2018 - Springer Verlag
    Colour vision depends on sufficient ambient light and becomes ineffective at a particular low light intensity. It is not known how decapod crustaceans see colour in dim light. In the present study we investigated the colour vision threshold in larvae of the giant freshwater prawn Macrobrachium rosenbergii in a tank under natural illumination. Plastic beads of different colours (blue, red, yellow and white) in various combinations were suspended in the tank. The larvae swam straight toward the beads and gathered around them. The number of larvae was highest on the blue and white beads. The luminance in the tank was then gradually decreased by covering it with different numbers of layers of black cloth, and the response of the larvae to the beads was video-recorded under infrared illumination. The preference for blue and white beads remained manifest as the luminance was reduced from 418 to 0.07 cd/m2, but not at 0.02 cd/m2, indicating a colour vision threshold between 0.07 and 0.02 cd/m2. The larvae have apposition compound eyes with large optical parameters, comparable to those of apposition eyes of nocturnal insects, which presumably capture more light and show enhanced sensitivity, enabling the larvae to see colour in dim light.
  • Article

    Characterisation of Vibrio isolates recovered from the eyes of cage-cultured pompano (Trachinotus blochii) infested with caligid parasites (Lepeophtheirus spinifer) 

    R Pakingking Jr., NB Bautista, D Catedral & EG de Jesus-Ayson - European Association of Fish Pathologists Bulletin, 2018 - European Association of Fish Pathologists
    Exophthalmia was documented among sea cage-cultured pompano (Trachinotus blochii) broodstocks with caligid parasite (Lepeophtheirus spinifer) infestation in the Philippines. Following sequencing, and based on the results of both diagnostic investigations and infection experiments, V. harveyi likely had a role in the reported exophthalmia cases, and this was initiated by L. spinifer infection.
  • Article

    Disaster management practices of academic libraries in Panay island, Philippines: Lessons from typhoon Haiyan 

    DL Superio, SB Alayon & MGH Oliveros - Information Development, 2018 - SAGE Publications
    A disaster management plan is essential because it can guide library personnel on what to do in a critical time. Using an eight-part survey instrument, this paper documents the impact of Typhoon Haiyan on 22 academic libraries in Northern Panay, Western Visayas, Philippines and the disaster management practices that were implemented. The results revealed that although the majority of the libraries do not have a disaster management plan, they all had common disaster management practices that enabled them to save parts of their collections. Moreover, the study revealed that librarians lacked knowledge and skills on disaster management. This paper was presented at the 40th International Association of Aquatic and Marine Science Libraries and Information Centers (IAMSLIC) Annual Conference, Noumea, New Caledonia, September 14–18, 2014.

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